<p><span style="font-size:14px"><span style="font-family:comic sans ms,cursive">RNA-seq, also called whole transcriptome shotgun sequencing (WTSS), usese next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment in time. RNA-seq is used to analyze the continually changing cellular transcriptome. Specifically, RNA-seq facilitates the ability to look at alternative gene spliced transcripts, post-transcriptional modifications, gene fusion, mutasions/SNPs and changes in gene expression. In addition to mRNA transcripts, RNA-seq can look at different populations of RNA to include total RNA, small RNA, such as miRNA, tRNA, and ribosomal profiling. RNA-seq cna also be used to determine exon/intron boundaries and verify or amend previously annotated 5&#39; and 3&#39; gene boundaries.</span></span></p>

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