Next generatioin seqeuncing
An Introduction to next-generation sequencing technology : http://www.illumina.com/content/dam/illumina-marketing/documents/products/illumina_sequencing_introduction.pdf
By Youtube
1) Next Generation Sequencing (NGS) - An Introduction
- NGS : sample prepartion->sequencing machines->DATA output by each cluster.
- four main DNA sequencing : pyrosequencing(release of pyrophosphate), sequencing by synthesis(fluorescent signal), sequencing by ligation(hybridization probe + oligonucleotide with fluorescent), ion semiconductor sequencing(Hydrogen ion releasing)
-https://www.youtube.com/watch?v=jFCD8Q6qSTM
2) Next Generation Sequencing (NGS) - Sample Preparation
- sample preparartion for whole Genome seq, exome seq, RNA seq, Methylation seq
- https://www.youtube.com/watch?v=-kTcFZxP6kM
3) Next Generation Sequencing (NGS) - Coverage & Sample Quality Control
- coverage(per genome = total output generated/total size of the sample seq), sample quality control, quantitative validation, qualitative validation.
- https://www.youtube.com/watch?v=PGAfwSRYv1g
4) Next Generation Sequencing (NGS) - Data Analysis
- Raw data output, sequence alignment, variant calling, additional software & tools. FASTQ, SAM.BAM, FASTQC, Picard
- https://www.youtube.com/watch?v=l4BAfRekohk
5) Next Generation Sequencing (NGS) - Applications of Whole Genome Sequencing
- WGS overview, De novo genome assembly, pathogen outbreak, molecular evolution.
