View source for Next generatioin seqeuncing

<p>An Introduction to next-generation sequencing technology&nbsp;:&nbsp;<a href="http://www.illumina.com/content/dam/illumina-marketing/documents/products/illumina_sequencing_introduction.pdf%3C/p%3E" rel="nofollow">http://www.illumina.com/content/dam/illumina-marketing/documents/products/illumina_sequencing_introduction.pdf</a></p>

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<p>By Youtube</p>

<p>1) Next Generation Sequencing (NGS) - An Introduction</p>

<p>- NGS&nbsp;: sample prepartion-&gt;sequencing machines-&gt;DATA output by each cluster.</p>

<p>- four main DNA sequencing&nbsp;: pyrosequencing(release of pyrophosphate), sequencing by synthesis(fluorescent signal), sequencing by ligation(hybridization probe + oligonucleotide with fluorescent), ion semiconductor sequencing(Hydrogen ion releasing)</p>

<p>-<a href="https://www.youtube.com/watch?v=jFCD8Q6qSTM%3C/p%3E" rel="nofollow">https://www.youtube.com/watch?v=jFCD8Q6qSTM</a></p>

<p>2) Next Generation Sequencing (NGS) - Sample Preparation</p>

<p>- sample preparartion for whole Genome seq, exome seq, RNA seq, Methylation seq</p>

<p>-&nbsp;<a href="https://www.youtube.com/watch?v=-kTcFZxP6kM%3C/p%3E" rel="nofollow">https://www.youtube.com/watch?v=-kTcFZxP6kM</a></p>

<p>3) Next Generation Sequencing (NGS) - Coverage &amp; Sample Quality Control</p>

<p>- coverage(per genome = total output generated/total size of the sample seq), sample quality control, quantitative validation, qualitative validation.</p>

<p>-&nbsp;<a href="https://www.youtube.com/watch?v=PGAfwSRYv1g%3C/p%3E" rel="nofollow">https://www.youtube.com/watch?v=PGAfwSRYv1g</a></p>

<p>4) Next Generation Sequencing (NGS) - Data Analysis</p>

<p>- Raw data output, sequence alignment, variant calling, additional software &amp; tools. &nbsp; &nbsp; FASTQ, SAM.BAM, FASTQC, Picard</p>

<p>-&nbsp;<a href="https://www.youtube.com/watch?v=l4BAfRekohk%3C/p%3E" rel="nofollow">https://www.youtube.com/watch?v=l4BAfRekohk</a></p>

<p>5) Next Generation Sequencing (NGS) - Applications of Whole Genome Sequencing</p>

<p>- WGS overview, De novo genome assembly, pathogen outbreak, molecular evolution.</p>

<p>-&nbsp;<a href="https://www.youtube.com/watch?v=bDsfd6V-U1c%3C/p%3E" rel="nofollow">https://www.youtube.com/watch?v=bDsfd6V-U1c</a></p>