Week2-sequencing

Week2-sequencing

 

                                                               20141215

Ji hoon moon

 

DNA sequencing process of determining the nucleotide order of a given DNA fragment. And it is most important things because they can analyze the human data codes. (genetic information). As we analyze the genetic information, we can research about more precisely and basic concepts of the living things.

 

First sequencing is developed by Sanger that synthesizes the new strand of one strand of certain DNA by using DNA polymerase and primer. This technique use sequence specific termination of a DNA synthesis reaction using modified nucleotide substrate.(A T G C)

And developed to shotgun method that are randomly generate sequence and analyze to the sequence information, pyrosequencing that use chemoluminesent

I will study about bioinformatics as a tool of genomics, After this essay I will write about bioinformatics study and submit at the last of the semester.

 

The sequence of DNA encodes the necessary information for living things to survive and reproduce. So we study about our genome, we can know about the ancestors and developmental trace about living things and if we use these information, we can do more useful things for human being. For example, analyze the genes of specific diseases, aging and so on.

And Determining the sequence is useful in fundamental biological research into why and how organisms live, what’s their means in universe. They can make us to study about the origin of universe. Because of the key importance DNA has to living things, knowledge of DNA sequences are useful in practically any area of biological research.

 

We usually know we can sequencing with RNA Protein DNA, But I dont know about how can we use that into tool for sequencing. So I wonder about it and have a search.

There aren’t nearly information but As a result, I can know about the ingredients of the sequencing that contains DNA information. For examples, Blood (whole blood, buffy coat, lymphocyte, plasma, serum), Tissue (Fresh,Frozen), FFPE (formalin-fixed, paraffin-embedded), Cultured cell, Bone marrow, FNA (Fine-needle aspiration), Buccal scrapes/swabs and mouthwash samples, Other (Semen, Urine, stool, saliva), So we can use that things to case specifically. 

 

In my opinion, the Other parts can use more useful when produce a service to customers that they want to genomic sequencing about themselves, having no hesitation of the customers( If we extract blood, customers don’t like needle or pain, they don’t like them)