1. Sequencing : Sanger, NGS, Methyl-seq -YJ code: 481887

From Biolecture.org

Sanger sequencing

 

What is the Sanger sequecing?

> Sanger sequencing is a method of DNA sequencing first commercialized by applied biosystems. Developed by FREderick Sanger and colleagues in 1977, it was the most widely used sequencing method for approximately 39 years. More recently, higher volume Sanger sequencing has been supplanted by NGS sequencing methods, especially for large-scale, automated genome analyses. However, the Sanger method remains in wide use, for smaller-scale projects, validation of Next-Gen results and for obtaining especially long contiguous DNA sequence reads.

 

How to sequecing?

> The classical chain-termination method requires a single-stranded DNA template, a DNA primer, a DNA polymerase, normal deoxynucleosidetriphosphates (dNTPs), and modified di-deoxynucleosidetriphosphates (ddNTPs), the latter of which terminate DNA strand elongation.

 

Reference : 

1. https://en.wikipedia.org/wiki/Sanger_sequencing

2. Sanger F; Coulson AR (May 1975). "A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase". J. Mol. Biol. 

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