Human Genomes - KPGP, KOREF

From Biolecture.org

[Korean Genome Project]

1) study Korean genome project.
2) learn about the difference between reference genome and re-sequenced genome.
3) tell how to use re-sequenced genome and reference genome.
4) how to assemble a reference genome.
5) What KOREF is.
6) How to get variants from reference genome.
7) The importance and application of KOREF.

KGP – Korean Genome Project

  • The Korean Genome Project is an open-free genome project that collects, analyzes, and distributes Korean genomes.
  • The second phase Korean Genome Project is also known as Korean Personal Genome Project (KPGP) as it is an open project for common people as well as scientists.
  • The first Korean Genome Project was sequencing and analyzing Dr. Kim Seong-Jin which is the first full Korean genome ever sequenced.
  • The second project was done by sequencing and analyzing AK1 genome.

 

KOREF – Korean Personal Genome Project

  • It is an independent sub-project of KGP.
  • It is taking the methodology of PGP (Personal Genome Project) as it is the most open and robust IRB (Institutional Review Board) based genome project for the public.
  • KGP has started as the national Korean Genome Project in KOBIC (국가생명연구자원정보센터), KRIBB (한국생명공학연구원), in 2006.

 

Reference genome & Re-sequenced genome

1) Reference genome: It is a digital nucleic acid sequence database, assembled by scientists as a representative example of a species’ set of genes. As they are often assembled from the sequencing of DNA from a number of donors, reference genomes do not accurately represent the set of genes of any single person. Instead a reference provides a haploid mosaic of different DNA sequences from each donor.

 

2) Re-sequenced genome: It enables the sequencing of selected segments of the genome in a large number of individuals. In using reference genome, there was a drawback that it can only allow deep genome-wide sequence coverage of a small number of individuals.

 

How to get variants from reference genome

The sequence data from each individual are aligned to the appropriate reference, and the genetic variants between the different samples can be detected as high-confidence sequence differences.

 

Importance & application of KOREF

Through sequencing as many as possible numbers of individual genomes, the genomic data pool will be made. And it will much efficiently improve the present medical technology. The diagnosis of disease ranging from flu to the rare disease would be easily possible. By enlarging the number of individual genomes, the more data accumulated, the more need of work have to be done and the more labor have to be involved. Thus it will provide more workplace which will lubricate the main social problem.

 

Reference

1. http://koreangenome.org/index.php/The_Korean_Genome_Project

2. http://www.nature.com/nbt/journal/v26/n1/full/nbt0108-65.html

3. https://en.wikipedia.org/wiki/Reference_genome
4. http://www.sciencedirect.com/science/article/pii/S0959437X06002085

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