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Scientific Experiment

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<p>pRA_GFP Digestion(EcoR1,Xho1) EcoR1 pRL mETP SpyTag Stop Xho1(3,5) Extraction</p>
 
<p>&nbsp;</p>
 
<p>&nbsp;(4)</p>
 
<p>EcoR1 pRL mETP SpyTag Stop Xho1 Tvector&amp;pRA_GFP_mETP&amp;pRA_GFP Digestion(EcoR1,Xho1)</p>
 
<p>[[File:18.png|400px]]</p>
 
<p>&nbsp;</p>
 
<p>(5)</p>
 
<p>&lt;Ligation&gt;</p>
 
<p>EcoR1_pRL_mETP_SpyTag_Stop_Xho1 T-Vector</p>
 
<p>-&gt; insert(9.3ng/ul): 1.5ul</p>
 
<p>-&gt; vector(19.3ng/ul): 3ul</p>
 
<p>-&gt; T4 DNA ligase Buffer: 1ul</p>
 
<p>-&gt; T4 DNA Enzyme: 1ul</p>
 
<p>-&gt; D.W: 3.5</p>
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
<p>&nbsp;</p>
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