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<p>pRA_GFP Digestion(EcoR1,Xho1) EcoR1 pRL mETP SpyTag Stop Xho1(3,5) Extraction</p>
<p> </p>
<p> (4)</p>
<p>EcoR1 pRL mETP SpyTag Stop Xho1 Tvector&pRA_GFP_mETP&pRA_GFP Digestion(EcoR1,Xho1)</p>
<p>[[File:18.png|400px]]</p>
<p> </p>
<p>(5)</p>
<p><Ligation></p>
<p>EcoR1_pRL_mETP_SpyTag_Stop_Xho1 T-Vector</p>
<p>-> insert(9.3ng/ul): 1.5ul</p>
<p>-> vector(19.3ng/ul): 3ul</p>
<p>-> T4 DNA ligase Buffer: 1ul</p>
<p>-> T4 DNA Enzyme: 1ul</p>
<p>-> D.W: 3.5</p>
<p> </p>
<p> </p>
<p> </p>