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<p> <b>GENOMICS</b></p>
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<hr /><p> <b> TRANSCRIPTOMICS</b></p><pol> <li>what is transcriptomics? transcript + omics. it is study of all set of RNAs including mRNA, rRNA, tRNA, so on. </pli> <li>relationship bewteen genomics. genome is the basis for the transcriptome. although only small portion of gene is transcribed (less than 5%[2]), it is useful to figure out the pattern of gene expression. especially multicellular organism, there are different type of cells having same genome, which is called genomic equivalence. transcription of genes is the first stage of gene expression, transcriptomic is the valuable source for understanding the gene expression of genome.</li> <li>what is mRNA? mRNA is the molecule carrying genetic information for protein synthesis. it is trasncripted from nucleus, exported to cytosol, and recognized by ribosome with its unique modification moiety such as 5'-cap and poly A tail.</li> <li>how to measure mRNA expression?</li> <li>relationship between trasncriptome & proteome. to cell function, a set of proteins are needed. Due to the fact that mRNA translates proteins it is easy to consider that linear correlation bewteen transcriptome and proteome, low correlation sometimes come up with the post translational modification[3]. but still transcriptomic is the middle step of understanding bewteen genetic code and the functioning molecule.</li> <li>what is UTR? UTR is abbreviation for untranslated region. it is the portion of mRNA which is not translated. there are two UTR - one is on the 5' end(5'-UTR), another on the 3' end(3'-UTR). those UTRs are related to modification and lifespan of mRNA. for example, polyadenylation is an addition of 200 ~ 300 adenine to 3' end of mRNA making poly A tail. this process depends on AATAAA sequence on the 3'-UTR.</li> <li>what is ncRNA?</li> <li>what is poly A? poly A is the long A sequence on the 3' end of mRNA. it is important for mRNA to fully function as genetic code for protein synthesis. there are 3 roles of poly A tail. 1) providing stability to mRNA, 2)recognizing mRNA for exporting from the nucleus or ribosome binding. 3)involving posttranscriptional modification by interacting with PABP(poly A binding protein), maskin, RISC, and so on.</li></ol>
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<p><b>REFERENCES</b></p>
<p>[1] http://www.merriam-webster.com/dictionary/lecture</p>
<p>[2] http://www.nature.com/scitable/topicpage/transcriptome-connecting-the-genome-to-gene-function-605#</p><p>[3] http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3637682/</p><p>[4] <span style="font-family: 'Open Sans', 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 14px; line-height: 21px; text-indent: -28px; background-color: rgb(242, 242, 242);">Gilbert, Scott F. </pspan><i style="box-sizing: border-box; font-family: 'Open Sans', 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 14px; line-height: 21px; text-indent: -28px; background-color: rgb(242, 242, 242);">Developmental Biology<p/i> <span style="font-family: 'Open Sans', 'Helvetica Neue', Helvetica, Arial, sans-serif; font-size: 14px; line-height: 21px; text-indent: -28px; background-color: rgb(242, 242, 242);">. Sunderland, MA: Sinauer Associates, 2010. Print.</span></p>
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