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<font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2">
<p align="left">Accurate whole human genome sequencing using reversible terminator chemistry<br />
<br />
<br />
They reported an approach that generates several billion bases of nucleotide sequence per experiment at a relatively low cost. Single molecules of DNA were attached to a flat surface, amplified </p>
</font></font></font><font face="AdvP4E9564" color="#292526" size="2"><font face="AdvP4E9564" color="#292526" size="2"><font face="AdvP4E9564" color="#292526" size="2">in situ </font></font></font><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2">and used as templates for synthetic sequencing with fluorescent reversible terminator deoxyribonucleotides. <br />
Images of the surface were analysed to generate high-quality sequence. They demonstrated the application of their approach to human genome sequencing on flow-sorted X chromosomes and then scaled the approach to determine the genome sequence of a male Yoruba from Ibadan, Nigeria. <br />
They built a consensus sequence from over </font></font></font><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2">30 fold</font></font></font><font face="AdvP7DA6" color="#292526" size="2"><font face="AdvP7DA6" color="#292526" size="2"><font face="AdvP7DA6" color="#292526" size="2"> </font></font></font><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2">average depth of paired 35-base reads. They characterized 4 million single-nucleotide polymorphisms and 4 hundred thousand structural variants, many of which were previously unknown. Their approach was reported to be effective for accurate, rapid, and economical whole-genome re-sequencing and many other biomedical applications.<br />
<br />
</font></font></font>
<p align="left">Accurate whole human genome sequencing using reversible terminator chemistry<br />
<br />
<br />
They reported an approach that generates several billion bases of nucleotide sequence per experiment at a relatively low cost. Single molecules of DNA were attached to a flat surface, amplified </p>
</font></font></font><font face="AdvP4E9564" color="#292526" size="2"><font face="AdvP4E9564" color="#292526" size="2"><font face="AdvP4E9564" color="#292526" size="2">in situ </font></font></font><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2">and used as templates for synthetic sequencing with fluorescent reversible terminator deoxyribonucleotides. <br />
Images of the surface were analysed to generate high-quality sequence. They demonstrated the application of their approach to human genome sequencing on flow-sorted X chromosomes and then scaled the approach to determine the genome sequence of a male Yoruba from Ibadan, Nigeria. <br />
They built a consensus sequence from over </font></font></font><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2">30 fold</font></font></font><font face="AdvP7DA6" color="#292526" size="2"><font face="AdvP7DA6" color="#292526" size="2"><font face="AdvP7DA6" color="#292526" size="2"> </font></font></font><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2"><font face="AdvP4E954F" color="#292526" size="2">average depth of paired 35-base reads. They characterized 4 million single-nucleotide polymorphisms and 4 hundred thousand structural variants, many of which were previously unknown. Their approach was reported to be effective for accurate, rapid, and economical whole-genome re-sequencing and many other biomedical applications.<br />
<br />
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