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<span style="font-size:14px;">Currently, I have completed the process of determining whether lipid nanoparticles (LNPs) can pass through the BBB. Now, I have shifted my focus to exploring the mechanisms by which these nanoparticles pass through the barrier. I became particularly interested in this area because I believe RNA-sequencing, which was mentioned in the Genomics course, would help me explore these mechanisms further. RNA-sequencing can provide detailed insights into the gene expression changes and molecular pathways activated during nanoparticle transport across the BBB, thereby elucidating the specific biological processes and interactions involved.</span>
<br/> <br/> <span style="font-size:14px;"><span style="line-height:107%">'''<span lang="EN-US">Blood-Brain Barrier and Lipid Nanoparticles</span>'''</span><br/> <span style="line-height:107%"><span lang="EN-US">The human blood-brain barrier (BBB) is a unique and selective barrier that regulates the transport of substances from Blood to the brain. It plays an important role in maintaining the neurons and glia function. BBB is composed of Brain microvascular endothermic cells (BMVECs), Astrocytes, Pericytes, and ECM (<span style="color:#0070c0">1,2</span>). BMVECs have a much lower degree of endocytosis/transcytosis activity than peripheral endothelium contributing to the characteristics of BBB as a barrier (<span style="color:#0070c0">3</span>).</span></span><br/> <span style="line-height:107%"><span lang="EN-US"> Lipid nanoparticles (LNPs) are widely used in the field of drug delivery. LNPs are primarily composed of cholesterol and lipids and can encapsulate DNA, RNA, or drugs. Additionally, ligands or antibodies can be attached to the surface to aid in targeting (<span style="color:#0070c0">4</span>).</span></span><br/> <span style="line-height:107%"><span lang="EN-US">My research is conducting a study on whether LNPs using a new fabrication method called Liposome Under Cryo-Assembly (Luca) can pass through the human BBB model. The identification of LNP samples that penetrate well is finished and the mechanism study process is in the process.</span></span></span><br/> <br/> <img style="width: 700px; height: 509px;" src=http://Biolecture.org/upload/20240611155233_image.png><br/> <span style="font-size:14px;">'''Figure1.'''<br/> The Timeline and Structure of Human BBB Transwell model.<br/> <br/> <span style="line-height:107%">'''<span lang="EN-US">Overview of RNA sequencing technology</span>'''</span><br/> <span style="line-height:107%"><span lang="EN-US">Principles of RNA Sequencing</span></span><br/> <span style="line-height:107%"><span lang="EN-US">RNA sequencing (RNA-seq) is a high-throughput sequencing technology used to determine the nucleotide sequence of RNA molecules and quantify specific RNA species within a population. The process of RNA sequencing includes the following steps (<span style="color:#0070c0">5</span>):</span></span></span>
#<span style="font-size:14px;"><span style="line-height:107%"><span lang="EN-US">RNA Extraction: Extracting RNA from biological samples such as cells or tissues.</span></span></span>
