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From Biolecture.org
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<p>DNA sequences are transcripted to mRNA and then translated to amino acids. During the translation, each three mRNA sequence is assigned to specific amino acid. In 2001 science, one interesting paper was published; Expanding genetic code of escherichia coli. In this paper, they generated unique tRNA/aminoacyl-tRNA synthetase pair to express non-canical amino acid at the site corresponed to amber codon in mRNA. In this way, they conducted in vivo incorporation O-methyl-tyrosine into protein in E-coli.</p>
<p><img alt="" src="/ckfinder/userfiles/images/%EA%B7%B8%EB%A6%BC2(1).jpg" style="height:51px154px; width:200px600px" /></p>
<p>Then why don't we improve this technique to express some unstable non-canonical amino acids?</p>
<p><strong>References</strong></p>
<p>Kim Wals and HuibOvaa, Front. Chem., 01 April 2014, Unnatural amino acid incorporation in E. coli</p>
<p>P. G. Besant, P. V. Attwood, M. J. Piggott. Current Protein & Peptide Science, Volume 10 , Issue 6 , 2009, Focus on Phosphoarginine and Phospholysine</p>
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