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<h3 style="margin-left: 80px;"><strong>Clonally amplified templates.</strong> most imaging systems have<br />
not been designed to detect single fluorescent events, so<br />
amplified templates are required. The two most common<br />
methods are <strong>emulsion PCR (emPCR)9 and solid-phase<br />
amplification</strong></h3>
<h3 style="margin-left: 80px;"><strong><img alt="" src="/ckfinder/userfiles/images/Picture3(1).png" style="height:97px; width:400px" /><u>emulsion PCR (emPCR)</u></strong></h3>
<p style="margin-left:80px"><strong><u><img alt="" src="/ckfinder/userfiles/images/Picture4(1).png" style="height:235px; width:400px" />solid-phase amplification </u></strong></p>
<p style="margin-left:80px"> </p>
<h3 style="margin-left: 40px;"><strong>Single-molecule templates.</strong> Although <strong>clonally amplified</strong><br />
methods offer certain advantages over bacterial cloning,<br />
some of the protocols are cumbersome to implement<br />
