Question Box

9/23    question

Regarding phylogenetic tree

My question: How can we know the divergence time?

-> We cannot know the exact divergence time so this tree can be wrong.

 We can just  compare their genes and roughly estimate it by their genetic difference.

Also we can use fossil record.

 

Jhong said it is a good question.

 

10/8    2 questions

Regarding DNA sequencing

My question: How do we know whether the sequence is right or wrong by linking fragments?

-> 1.Sequence genome multiple times to reduce error.

2. Experimentally we can seperate each DNA in a well then we know certain fragemnts are come from 1 DNA. And linking them.

 

Jhong said it is a good question.

 

My question: When using second method, we can know that they are all in one line but how can we control the distance between each fragment?

-> By using long sequence, computer can link them easily but if it is not, we can do pair-end sequencing.

By using long scaffold and fill the gap. If the gaps fill easily, it means it is accurate. Also, compare with other species's genome by using CEGMA or BUSCO.

 

Jhong said it is a good question.

 

10/14 Question and answering

Regarding the question that 'what topic or question would you make relating with vulture genomics?'

My answer:  their digestive system, which can digest rotten meat. Also their habitat because they can live in harsh environment.

 

Jhong said their digestive system could be good topic. In case of habitat, it also can be a topic but is not much related with human.

 

My question: If we inject certain genes to different species, that species can get same property that we inject?

-> It can. Because, most of sequence is similar although they are different species.

 

10/28 Question

Regarding oncomics, after seeing the data about genome squencing .

My question: 'Why men's cancer mortality is higher than women although Y chromosome does not occur mutation in that data? '

-> In case of men, they have X, Y chromosomes so, it can be because their X chromosome is unstable. 

(However, this is different that I estimated in my essay that I already had written. But Jhong also said it is his guess.)

 

11/11  2Answers

Regarding telomere sequence, 'Why the sequence is TTAGGG?'

My Answer:

It can be related with stop codon. Because, TAG among the TTAGGG sequence is stop codon and by using it as a template, AATCCC in different strand.

So, by the opposite direction, TAA is also stop codon.

 

Jhong said it is a good estimation. 

 

Regarding telomere length, in animal, if you have a longer telomere, it means die earlier. (By comparing mouse and human.) 

My answer:

Although the telomere length is longer but the cell cycle time can be different.

 

Jhong siad it could be a reason because, they can die early if the cell cycle time is short eventhough they have longer telomere.