Seongwan's Transcriptomics
Contents
Definition
Transcriptomics is the study of the transcriptome—the complete set of RNA transcripts that are produced by the genome, under specific circumstances or in a specific cell
Relation between genomics and transcriptomics
Now, we can simply imagine 'central dogma'. DNA is replicated and it can be used as a template for transcription. mRNA is used as a template for translation.
The transcriptome is the product of the transcription. Therefore, genomics and transcriptomics are very related to each other. Basically, RNA is complementary to DNA.
However, RNA undergoes many different procedure, it gets its own properties.
classification of RNA molecule
mRNA
Messenger RNA (mRNA) is a large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acidsequence of the protein products of gene expression. Following transcription of primary transcript mRNA (known as pre-mRNA) by RNA polymerase, processed, mature mRNA is translated into a polymer of amino acids
UTR
In molecular genetics, an untranslated region (or UTR) refers to either of two sections, one on each side of a coding sequence on a strand of mRNA. If it is found on the 5' side, it is called the 5' UTR (or leader sequence), or if it is found on the 3' side, it is called the 3' UTR (or trailer sequence).
poly A
Polyadenylation is the addition of a poly(A) tail to a messenger RNA. The poly(A) tail is important for the nuclear export, translation, and stability of mRNA. The tail is shortened over time, and, when it is short enough, the mRNA is enzymatically degraded.
ncRNA
A non-coding RNA (ncRNA) is a functional RNA molecule that is not translated into a protein. Non-coding RNA genes include highly abundant and functionally important RNAs such as transfer RNAs (tRNAs) and ribosomal RNAs(rRNAs), as well as RNAs such as microRNAs, siRNAs, and snRNAs.
measurment of RNA expression
RNA-seq is emerging (2013) as the method of choice for measuring transcriptomes of organisms, though the older technique of DNA microarrays is still used.
