Transcriptomics Class

[ Transcriptomics class ] 

 

# Transcriptome? 

  = set of all mRNA molecules that are produced by the gemone, under specific circumstances in one cell or a population of cells 

  = can vary depending on environmental conditions

     ⇒ reflects genes which are being actively expressed with the exception of mRNA degradation 

# Transcriptomics?

  = the study of transcriptome 

  = referred to as expression profiling

  = examines the expression level of mRNAs in a given cell population, often using high-throughput techniques based on DNA microarray technology. 

2. Relationship between genomics and transcriptomics

3. What are mRNAs?

4. How to measure RNA expression?

5. Relationship between Transcriptome and Proteome.

6. What is a UTR?

7. What is ncRNA ?

8. What is poly A ?

 

 

What to do:

1. Apply Biology learning procedure

 

 

Reference

http://www.biomedcentral.com/1471-2164/13/473

https://www.youtube.com/watch?v=UYJfM2-Y7y8  (Youtube by Illumina Gary Schroth)

 

 

 

Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNA-Seq

Kyung-Do Park^1†, Jongsun Park^2†, Junsu Ko^3†, Byung Chul Kim²³, Heui-Soo Kim⁴, Kung Ahn⁴, Kyoung-Tag Do⁵, Hansol Choi², Hak-Min Kim², Sanghoon Song³, Sunghoon Lee², Sungwoong Jho², Hong-Sik Kong¹, Young Mok Yang⁶, Byung-Hak Jhun⁷, Chulhong Kim³, Tae-Hyung Kim³, Seungwoo Hwang⁸, Jong Bhak^23*, Hak-Kyo Lee^1* and Byung-Wook Cho^5*