Dahyun week12

Fluorescent In Situ Hybridization(FISH)

DNA sequencing utilizing fluorescent markers is a method to analyze genetic sequences and visualize specific genes or genomic regions by attaching fluorescent dyes to specific bases. One of the most representative methods includes the following procedure:  

1. Use of Fluorescent Markers: Each DNA base (A, T, G, C) is tagged with a different fluorescent marker (color). As a result, each base emits light of a specific wavelength, giving it a unique color.
2. Sequencing Process: During DNA sequencing, the sequence of a specific DNA fragment is determined. Each DNA base amplified or replicated in the sequencing reaction is identified by the color of light emitted by the fluorescent marker attached to it.
3. Image Capture and Analysis: The light emitted by the fluorescent markers is captured using a special camera or detector. This data is analyzed by computer software, determining the DNA sequence based on the colors of the fluorescent markers observed at each position.
4. Sequencing Results: Ultimately, the DNA sequence is represented by the order of fluorescent colors. This allows researchers to identify specific areas of the genome, genetic mutations, or genetic changes related to diseases.

ref: https://www.youtube.com/watch?v=MvuYATh7Y74