About sequencing

Sequencing is consist of three steps

 

1. [Library preparation]

2. [Template preparation]

3. [Sequencing and imaging]

 

Library preparation

A genomic library is a collection of the total genomic DNA from a single  organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size.

 

 

 

 

Template preparation

 

 

Sequencing and imaging