Alignment S
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Revision as of 19:46, 28 November 2016 by imported>S (Created page with "<p>-NCBI Blast tutorial : https://www.youtube.com/watch?v=HXEpBnUbAMo</p> <p>- Bioinformatics practical 4 multiple sequence alignment using ClustalW : https://www...")
-NCBI Blast tutorial : https://www.youtube.com/watch?v=HXEpBnUbAMo
- Bioinformatics practical 4 multiple sequence alignment using ClustalW : https://www.youtube.com/watch?v=tq-bWYVnQCM
1. Find the protein in NCBI and retrieve the sequence and save it in computer
2. Run BLAST to find similar sequences and retrieve some similar sequences in FASTA format
3. Look for the presence of conseved domain
4. Go for the multiple sequence alignment with 10 of the retrieved sequence
5. Construct the phylogenetic tree with the sequence alignment.
6. Predict the secondary structure of the protein
7. Retrieved the 3D structure of protein from PDB
8. View the 3D structure of protein in Jmol
amino acid table
- clustal W : https://www.ebi.ac.uk/Tools/msa/clustalo/
