Chapter !10 - Proteomics Code : KSI0019

<Index of Chapter 10>

 

Introduction

Protein nature and types

Protein structure

-The chemical structure of proteins 

-Conformation of the polypeptide chain

-Protein folding patterns

Post-translational modifications

-Why is there a common genetic code with 20 canorical amino acids?

Seperation and anylsis of proteins 

-Polyacrylamide gel electrophoresis (PAGE)

-Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)

-Mass spectrometry

Classification of protein structures

-SCOP

-Changes in folding patterns in protein evolution

Many proteins change conformation as part of the mechanism of their function

-Conformational change during enzymatic catalysis

-Motor proteins

-Allosteric regulation of protein function

-Conformational states of serine protease inhibitors (serpins)

Protein structure prediction and modelling

-Homology modelling

-Available protocols for protein structure prediction

-Structural genomics

Directed evolution and protein design

-Directed evolution of subtilisin E

-Enzyme design

Protein complexes and aggregates

-Protein aggregation diseases

-Properties of protein-protein complexes

-Multisubinit proteins