<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">1.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">What is transcriptomics?<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Thranscriptomics is discipline of biology that study about the set of all RNA moleculs, including mRNA, rRNA, tRNA and non-coding RNA transcribed in one cell or a population of cells<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">2.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Relationship between genomics and transcriptomics<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Transcriptomics is expressional genomics. <o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">3.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">What are mRNAs?<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">mRNA is large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence if the protein products of gene expressing<o:p></o:p></span></p>
<p class="MsoListParagraph" style="margin: 0cm 0cm 8pt 40pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black; line-height: 107%"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">4.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">How to measure RNA expression?<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif">RNA-seq method is frequently used for measuring RNA expression.<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">5.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Relationship between Transcriptome and Proteome.<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt; line-height: 14.3pt; text-indent: 20.25pt; mso-char-indent-count: 1.5"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Proteome is expressional Transcriptome. <o:p></o:p></span></p>
<p class="MsoListParagraph" style="margin: 0cm 0cm 8pt 40pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black; line-height: 107%"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">6.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">What is a UTR?<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white; mso-bidi-font-weight: bold">Untranslated region </span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white">(or<span class="apple-converted-space"> </span><span style="mso-bidi-font-weight: bold">UTR</span>) refers to either of two sections, one on each side of a<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="Coding sequence" href="http://en.wikipedia.org/wiki/Coding_sequence"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; text-underline: none">coding sequence</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white"> </span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white">on a strand of<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="MRNA" href="http://en.wikipedia.org/wiki/MRNA"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; text-underline: none">mRNA</span></a></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white">. If it is found on the 5' side, it is called the<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="Five prime untranslated region" href="http://en.wikipedia.org/wiki/Five_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; text-underline: none">5' UTR</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white"> </span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white">(or<span class="apple-converted-space"> </span><span style="mso-bidi-font-weight: bold">leader sequence</span>), or if it is found on the 3' side, it is called the<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="Three prime untranslated region" href="http://en.wikipedia.org/wiki/Three_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; text-underline: none">3' UTR</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white"> </span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white">(or<span class="apple-converted-space"> </span><span style="mso-bidi-font-weight: bold">trailer sequence</span>).<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">7.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">What is ncRNA ?<o:p></o:p></span></p>
<p class="MsoNormal" style="margin: 0cm 0cm 8pt 15pt; mso-para-margin-left: 1.5gd"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white; line-height: 107%">A<span class="apple-converted-space"> </span><span style="mso-bidi-font-weight: bold">non-coding RNA</span><span class="apple-converted-space"> </span>(<span style="mso-bidi-font-weight: bold">ncRNA</span>) is a functional<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="RNA" href="http://en.wikipedia.org/wiki/RNA"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; line-height: 107%; text-underline: none">RNA</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white; line-height: 107%"> </span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white; line-height: 107%">molecule that is not<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="Translation (genetics)" href="http://en.wikipedia.org/wiki/Translation_(genetics)"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; line-height: 107%; text-underline: none">translated</span></a></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; background: white; line-height: 107%"> into a<span class="apple-converted-space"> </span></span><span lang="EN-US"><a title="Protein" href="http://en.wikipedia.org/wiki/Protein"><span style="font-size: 13.5pt; text-decoration: none; font-family: "Arial",sans-serif; background: white; color: windowtext; line-height: 107%; text-underline: none">protein</span></a></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; line-height: 107%"><o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: "Arial",sans-serif; color: black"><o:p> </o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">8.<span style="font: 7pt "Times New Roman""> </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">What is poly A?<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: "Arial",sans-serif; color: black">Poly A is long tail sequence of adenine nucleotides add it the 3’end of the pre-mRNA. This promotes export from the nucleus and translation, and protects the mRNA from degradation<o:p></o:p></span></p>
<p class="MsoNormal" style="margin: 0cm 0cm 8pt"><span lang="EN-US"><o:p><font size="2" face="맑은 고딕"> </font></o:p></span></p>
<p> </p>
<ol style="list-style-type: decimal; direction: ltr">
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<div style="margin-bottom: 6pt; font-size: 12pt; background: white; font-weight: normal; color: rgb(0,0,0); font-style: normal; margin-top: 4.8pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">What is transcriptomics?</span></div>
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<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Thranscriptomics is discipline of biology that study about the set of all RNA moleculs, including mRNA, rRNA, tRNA and non-coding RNA transcribed in one cell or a population of cells</span></p>
<div style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </div>
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<div style="margin-bottom: 6pt; font-size: 12pt; background: white; font-weight: normal; color: rgb(0,0,0); font-style: normal; margin-top: 4.8pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Relationship between genomics and transcriptomics</span></div>
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<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Transcriptomics is expressional genomics. </span></p>
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div>
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<div style="margin-bottom: 6pt; font-size: 12pt; background: white; font-weight: normal; color: rgb(0,0,0); font-style: normal; margin-top: 4.8pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">What are mRNAs?</span></div>
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<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">mRNA is large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence if the protein products of gene expressing</span></p>
<div style="margin: 0cm 0cm 8pt 40pt"> </div>
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div>
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<div style="margin-bottom: 6pt; font-size: 12pt; background: white; font-weight: normal; color: rgb(0,0,0); font-style: normal; margin-top: 4.8pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">How to measure RNA expression?</span></div>
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<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span style="font-size: 13.5pt; sans-serif: ">RNA-seq method is frequently used for measuring RNA expression.</span></p>
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div>
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div>
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<p style="margin-bottom: 6pt; font-size: 12pt; background: white; font-weight: normal; color: rgb(0,0,0); font-style: normal; margin-top: 4.8pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Relationship between Transcriptome and Proteome.</span></p>
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<div style="background: white; margin: 4.8pt 0cm 6pt; line-height: 14.3pt; text-indent: 20.25pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Proteome is expressional Transcriptome. </span></div>
<div style="margin: 0cm 0cm 8pt 40pt"> </div>
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div>
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<div style="margin-bottom: 6pt; font-size: 12pt; background: white; font-weight: normal; color: rgb(0,0,0); font-style: normal; margin-top: 4.8pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">What is a UTR?</span></div>
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<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; background: white; sans-serif: ">Untranslated region </span><span style="font-size: 13.5pt; background: white; sans-serif: ">(or UTR) refers to either of two sections, one on each side of a </span><a title="Coding sequence" href="http://en.wikipedia.org/wiki/Coding_sequence"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">coding sequence</span> <span style="font-size: 13.5pt; background: white; sans-serif: ">on a strand of </span><a title="MRNA" href="http://en.wikipedia.org/wiki/MRNA"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">mRNA</span><span style="font-size: 13.5pt; background: white; sans-serif: ">. If it is found on the 5' side, it is called the </span><a title="Five prime untranslated region" href="http://en.wikipedia.org/wiki/Five_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">5' UTR</span> <span style="font-size: 13.5pt; background: white; sans-serif: ">(or leader sequence), or if it is found on the 3' side, it is called the </span><a title="Three prime untranslated region" href="http://en.wikipedia.org/wiki/Three_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">3' UTR</span> <span style="font-size: 13.5pt; background: white; sans-serif: ">(or trailer sequence).</span></a></a></a></a></p>
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div>
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<div style="margin-bottom: 6pt; font-size: 12pt; background: white; font-weight: normal; color: rgb(0,0,0); font-style: normal; margin-top: 4.8pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">What is ncRNA ?</span></div>
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<div style="margin: 0cm 0cm 8pt 15pt"><span style="font-size: 13.5pt; background: white; line-height: 107%; sans-serif: ">A non-coding RNA (ncRNA) is a functional </span><a title="RNA" href="http://en.wikipedia.org/wiki/RNA"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; line-height: 107%; text-underline: none; sans-serif: ">RNA</span> <span style="font-size: 13.5pt; background: white; line-height: 107%; sans-serif: ">molecule that is not </span><a title="Translation (genetics)" href="http://en.wikipedia.org/wiki/Translation_(genetics)"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; line-height: 107%; text-underline: none; sans-serif: ">translated</span><span style="font-size: 13.5pt; background: white; line-height: 107%; sans-serif: "> into a </span><a title="Protein" href="http://en.wikipedia.org/wiki/Protein"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; line-height: 107%; text-underline: none; sans-serif: ">protein</span></a></a></a></div>
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div>
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div>
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div>
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div>
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<div style="margin-bottom: 6pt; font-size: 12pt; background: white; font-weight: normal; color: rgb(0,0,0); font-style: normal; margin-top: 4.8pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">What is poly A?</span></div>
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<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Poly A is long tail sequence of adenine nucleotides add it the 3’end of the pre-mRNA. This promotes export from the nucleus and translation, and protects the mRNA from degradation</span></p>
<div style="margin: 0cm 0cm 8pt"><font size="2"> </font></div>