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Trancriptomics Assay

14,282 bytes added, 00:34, 21 May 2015
Created page with "<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-fami..."
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">1.<span style="font: 7pt &quot;Times New Roman&quot;">&nbsp;&nbsp; </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">What is transcriptomics?<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">Thranscriptomics is discipline of biology that study about the set of all RNA moleculs, including mRNA, rRNA, tRNA and non-coding RNA transcribed in one cell or a population of cells<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">2.<span style="font: 7pt &quot;Times New Roman&quot;">&nbsp;&nbsp; </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">Relationship between genomics and transcriptomics<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">Transcriptomics is expressional genomics. <o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">3.<span style="font: 7pt &quot;Times New Roman&quot;">&nbsp;&nbsp; </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">What are mRNAs?<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">mRNA is large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence if the protein products of gene expressing<o:p></o:p></span></p>
<p class="MsoListParagraph" style="margin: 0cm 0cm 8pt 40pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black; line-height: 107%"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">4.<span style="font: 7pt &quot;Times New Roman&quot;">&nbsp;&nbsp; </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">How to measure RNA expression?<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif">RNA-seq method is frequently used for measuring RNA expression.<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">5.<span style="font: 7pt &quot;Times New Roman&quot;">&nbsp;&nbsp; </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">Relationship between Transcriptome and Proteome.<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt; line-height: 14.3pt; text-indent: 20.25pt; mso-char-indent-count: 1.5"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">Proteome is expressional Transcriptome. <o:p></o:p></span></p>
<p class="MsoListParagraph" style="margin: 0cm 0cm 8pt 40pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black; line-height: 107%"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">6.<span style="font: 7pt &quot;Times New Roman&quot;">&nbsp;&nbsp; </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">What is a UTR?<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white; mso-bidi-font-weight: bold">Untranslated region </span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white">(or<span class="apple-converted-space">&nbsp;</span><span style="mso-bidi-font-weight: bold">UTR</span>) refers to either of two sections, one on each side of a<span class="apple-converted-space">&nbsp;</span></span><span lang="EN-US"><a title="Coding sequence" href="http://en.wikipedia.org/wiki/Coding_sequence"><span style="font-size: 13.5pt; text-decoration: none; font-family: &quot;Arial&quot;,sans-serif; background: white; color: windowtext; text-underline: none">coding sequence</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white">&nbsp;</span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white">on a strand of<span class="apple-converted-space">&nbsp;</span></span><span lang="EN-US"><a title="MRNA" href="http://en.wikipedia.org/wiki/MRNA"><span style="font-size: 13.5pt; text-decoration: none; font-family: &quot;Arial&quot;,sans-serif; background: white; color: windowtext; text-underline: none">mRNA</span></a></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white">. If it is found on the 5' side, it is called the<span class="apple-converted-space">&nbsp;</span></span><span lang="EN-US"><a title="Five prime untranslated region" href="http://en.wikipedia.org/wiki/Five_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; font-family: &quot;Arial&quot;,sans-serif; background: white; color: windowtext; text-underline: none">5' UTR</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white">&nbsp;</span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white">(or<span class="apple-converted-space">&nbsp;</span><span style="mso-bidi-font-weight: bold">leader sequence</span>), or if it is found on the 3' side, it is called the<span class="apple-converted-space">&nbsp;</span></span><span lang="EN-US"><a title="Three prime untranslated region" href="http://en.wikipedia.org/wiki/Three_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; font-family: &quot;Arial&quot;,sans-serif; background: white; color: windowtext; text-underline: none">3' UTR</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white">&nbsp;</span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white">(or<span class="apple-converted-space">&nbsp;</span><span style="mso-bidi-font-weight: bold">trailer sequence</span>).<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">7.<span style="font: 7pt &quot;Times New Roman&quot;">&nbsp;&nbsp; </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">What is ncRNA&nbsp;?<o:p></o:p></span></p>
<p class="MsoNormal" style="margin: 0cm 0cm 8pt 15pt; mso-para-margin-left: 1.5gd"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white; line-height: 107%">A<span class="apple-converted-space">&nbsp;</span><span style="mso-bidi-font-weight: bold">non-coding RNA</span><span class="apple-converted-space">&nbsp;</span>(<span style="mso-bidi-font-weight: bold">ncRNA</span>) is a functional<span class="apple-converted-space">&nbsp;</span></span><span lang="EN-US"><a title="RNA" href="http://en.wikipedia.org/wiki/RNA"><span style="font-size: 13.5pt; text-decoration: none; font-family: &quot;Arial&quot;,sans-serif; background: white; color: windowtext; line-height: 107%; text-underline: none">RNA</span></a></span><span class="apple-converted-space"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white; line-height: 107%">&nbsp;</span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white; line-height: 107%">molecule that is not<span class="apple-converted-space">&nbsp;</span></span><span lang="EN-US"><a title="Translation (genetics)" href="http://en.wikipedia.org/wiki/Translation_(genetics)"><span style="font-size: 13.5pt; text-decoration: none; font-family: &quot;Arial&quot;,sans-serif; background: white; color: windowtext; line-height: 107%; text-underline: none">translated</span></a></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; background: white; line-height: 107%"> into a<span class="apple-converted-space">&nbsp;</span></span><span lang="EN-US"><a title="Protein" href="http://en.wikipedia.org/wiki/Protein"><span style="font-size: 13.5pt; text-decoration: none; font-family: &quot;Arial&quot;,sans-serif; background: white; color: windowtext; line-height: 107%; text-underline: none">protein</span></a></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; line-height: 107%"><o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 9.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black"><o:p>&nbsp;</o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt; text-indent: -18pt; mso-list: l0 level1 lfo1"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black; mso-fareast-font-family: Arial"><span style="mso-list: Ignore">8.<span style="font: 7pt &quot;Times New Roman&quot;">&nbsp;&nbsp; </span></span></span><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">What is poly A?<o:p></o:p></span></p>
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span lang="EN-US" style="font-size: 13.5pt; font-family: &quot;Arial&quot;,sans-serif; color: black">Poly A is long tail sequence of adenine nucleotides add it the 3&rsquo;end of the pre-mRNA. This promotes export from the nucleus and translation, and protects the mRNA from degradation<o:p></o:p></span></p>
<p class="MsoNormal" style="margin: 0cm 0cm 8pt"><span lang="EN-US"><o:p><font size="2" face="맑은 고딕">&nbsp;</font></o:p></span></p>
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