<br/> <br/> <span style="font-size:14px;"><span style="line-height:107%">'''<span lang="EN-US">Necessity of RNA Sequencing in BBB Models</span>'''</span><br/> <span style="line-height:107%"><span lang="EN-US">Through fluorescence image analysis, I found out that LNPs migrate to the transcytosis pathway. In addition, I checked the difference in transmittance by performing several transcytosis inhibitor treatments.</span></span><br/> <span style="line-height:107%"><span lang="EN-US">Through RNA sequencing, I would like to check whether the permeability has changed due to changes in the genetic level of the cells that actually make up BBB.</span></span><br/> <br/> <span style="line-height:107%">'''<span lang="EN-US">Future Plan</span>'''</span><br/> <span style="line-height:107%"><span lang="EN-US">Listening Taking to the Genomics course, I thought that conducting research at various gene levels was essential to conduct research at a high level. Later, the goal is to proceed with the RNA sequencing mentioned above to find out the detailed mechanisms that have not yet been found. After finding out the mechanism, I will treat the Aptamer on the surface to find out the change in the permeability and put the actual drug inside to deliver it.</span></span><br/> <br/> <br/> <span style="line-height:107%">'''<span lang="EN-US">References</span>'''</span><br/> <span style="line-height:107%"><span lang="EN-US" style="line-height:107%">(1) Cecchelli, Romeo, et al., Modelling of the blood–brain barrier in drug discovery and development., '''2007''', ''Nature Reviews Drug Discovery''. vol. 6, no. 8, pp. 650–661,.</span></span><br/> <br/> <span style="line-height:107%"><span lang="EN-US" style="line-height:107%">(2) Park, Tae-Eun, et al., Hypoxia-enhanced blood-brain barrier chip recapitulates human barrier function and shuttling of drugs and antibodies., '''2019''', ''Nature Communications'', vol. 10, no. 1, 13</span></span><br/> <br/> <span style="line-height:107%"><span lang="EN-US" style="line-height:107%">(3) Abbott, N. Joan, Lars Rönnbäck, and Elisabeth Hansson., Astrocyte–Endothelial Interactions at the Blood–Brain Barrier., '''2006''', ''Nature Reviews Neuroscience 7'', no. 1: 41–53.</span></span><br/> <br/> <span style="line-height:107%"><span lang="EN-US" style="line-height:107%">(4) Mehta, M., Bui, T. A., Yang, X., Aksoy, Y., Goldys, E. M., & Deng, W., Lipid-based nanoparticles for drug/gene delivery: An overview of the production techniques and difficulties encountered in their industrial development., '''2023''', ''ACS Materials Au'', 3(6), 600–619.</span></span><br/> <br/> <span style="line-height:107%"><span lang="EN-US">(5)</span> <span lang="EN-US">Deshpande, Dhrithi, et al., RNA-seq data science: From RAW data to effective interpretation.,'''2023''', ''Frontiers in Genetics'', vol. 14,</span></span></span><br/> &nbsp;