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<p>This problem has been addressed with the use of modified DNA polymerase enzyme systems and dyes that minimize incorporation variability, as well as methods for eliminating "dye blobs". The dye-terminator sequencing method, along with automated high-throughput DNA sequence analyzers, was used for the vast majority of sequencing projects until the introduction of Next Generation Sequencing.</p>
<h3>Automation and sample preparation[<a href="https://en.wikipedia.org/w/index.php?title=Sanger_sequencing&action=edit&section=3" title="Edit section: Automation and sample preparation">edit</a>]</h3>
<p><a href="https://en.wikipedia.org/wiki/File:Sanger_sequencing_read_display.png"><img alt="" src="https://upload.wikimedia.org/wikipedia/commons/thumb/9/98/Sanger_sequencing_read_display.png/220px-Sanger_sequencing_read_display.png" style="height:51px; width:220px" /></a></p>
<p>A polymerase sequences the complementary strand on top of the arched strand. They separate, and the 3' end of each strand is blocked. The forward strand is washed away, and the process of sequence by synthesis repeats for the reverse strand.</p>
<h3>Data analysis[<a href="https://en.wikipedia.org/w/index.php?title=Illumina_dye_sequencing&action=edit&section=8" title="Edit section: Data analysis">edit</a>]</h3>
<p>The sequencing occurs for millions of clusters at once, and each cluster has ~1,000 identical copies of a DNA insert.<sup><a href="https://en.wikipedia.org/wiki/Illumina_dye_sequencing#cite_note-Morozova-10">[10]</a></sup> The sequence data is analyzed by finding fragments with overlapping areas, called <a href="https://en.wikipedia.org/wiki/Contig" title="Contig">contigs</a>, and lining them up. If a reference sequence is known, the contigs are then compared to it for variant identification.</p>