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<p style="margin-left:40px"><img alt="" src="/ckfinder/userfiles/images/Picture2(1).png" style="height:13px; width:200px" /></p>
<p style="margin-left: 80px;">Sequencing technologies include a number of methods<br />
that are grouped broadly as <strong>template preparation,<br />
sequencing and imaging, and data analysis.</strong></p>
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<p><em>There are two methods used in preparing templates for NGS reactions</em>: <strong>clonally amplified templates</strong> originating from single DNA molecules, and<strong> single DNA molecule templates</strong></p>
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<pstyle="margin-left: 80px;"><strong>Clonally amplified templates.</strong> most imaging systems have<br />not been designed to detect single fluorescent events, so<br />amplified templates are required. The two most common<br />methods are <strong>emulsion PCR (emPCR)9 and solid-phase<br />amplification</strong></p> <p style="margin-left: 80px;"><strong><img alt="" src="/ckfinder/userfiles/images/Picture3(1).png" style="height:97px; width:400px" /><u>emulsion PCR (emPCR)</u></strong></p> <p style="margin-left: 80px;"><strong><u><img alt="" src="/ckfinder/userfiles/images/Picture4(1).png" style="height:235px; width:400px" />solid-phase amplification</u></strong></p> <p style="margin-left: 80px;"> </p>
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