Polony DNA sequencing. 2006 Curr Protoc Mol Biol.

Polony DNA sequencing.


Porreca GJ, Shendure J, Church GM.

Curr Protoc Mol Biol. 2006 Nov;Chapter 7:Unit 7.8.

Harvard Medical School, Boston, Massachusetts, USA.

Polony DNA sequencing provides an inexpensive, accurate, high-throughput way to resequence genomes of interest by comparison to a reference genome. Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on a microscope slide. Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences.

PMID: 18265387 [PubMed - indexed for MEDLINE]

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