<p><span style="font-family:times new roman,times,serif"><span style="font-size:22px"><strong>Sequencing</strong></span></span></p>

<p><span style="font-size:14px"><span style="font-family:times new roman,times,serif"><u><strong>Definition</strong></u></span></span></p>

<p><span style="font-size:14px"><span style="font-family:times new roman,times,serif">&nbsp; Sequencing is an analysis of biopolymer such as DNA, RNA, and protein for finding out the exact structure (atomic-level). RNA sequencing is a process of ordering and lining the nucleotides in given structure. Although the RNA information can be acquired by observing DNA sequence, it has its own meaning. Protein sequencing can be conducted in various way, Edman degradation, Peptide mass fingerprinting, Protease digests, and Mass spectrometry. Through protein sequencing, it can be helpful to sequence target DNA by inferring the protein information.</span></span></p>

<p><span style="font-size:14px"><span style="font-family:times new roman,times,serif"><u><strong>DNA Sequencing</strong></u></span></span></p>

<p><span style="font-size:14px"><span style="font-family:times new roman,times,serif">&nbsp; As the methods are keep developed, in these days, the most genome data is analyzed more by pyrosequencing (or 454 sequencing) than Sanger sequencing. It enables faster analysis by using single-nucleotide addition (SNA) method. Due to the limitation in the numbers of nucleotides that can be paused during DNA elongation, the strands can be extended by adding more single nucleotides. And each nucleotide which is linked on the strand adds fluorescence gradually. The increment of its signal can be analyzed in the form of program, which shows the order of lettered nucleotides.</span></span></p>

<p><span style="font-size:14px"><span style="font-family:times new roman,times,serif"><u><strong>Human Genome Project</strong></u></span></span></p>

<p><span style="font-size:14px"><span style="font-family:times new roman,times,serif">&nbsp; Using those methods described above, many stages of Human Genome Project is completed successfully. With present information, much advanced biomedical research is possible. But still millions of repeat-rich heterochromatin and many significant gaps in DNA strands are not determined.</span></span></p>

<p><span style="font-size:14px"><span style="font-family:times new roman,times,serif"><u><strong>Conclusion</strong></u></span></span></p>