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ShinUnBeom

171 bytes added, 02:38, 15 May 2015
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<p>&nbsp;Genomics is discipline of genetics for sequencing, assembling and analyzing the function and structure of entire&nbsp;genomes, not single genes.</p>
<h3>Genome analysis</h3>
<h4h5>DNA sequencing</h4h5>
<p>&nbsp;First of all, select an organism of interest. Then sequence DNA fragments. There are several ways for sequencing DNA fragments; shotgun sequencing[http://en.wikipedia.org/wiki/Shotgun_sequencing], high-throughout sequencing, illumina sequencing[http://en.wikipedia.org/wiki/Illumina_dye_sequencing] and ion torrent[http://en.wikipedia.org/wiki/Ion_semiconductor_sequencing].</p>
<h4h5>Sequence assembly</h4h5>
<p>&nbsp;After sequencing DNA fragments, next step is assembling DNA fragments to rebuild original chromosome. There are two ways; de-novo assembly[http://en.wikipedia.org/wiki/De_novo_transcriptome_assembly] which assemble without&nbsp;any reference sequences, and comparative assembly which use&nbsp;sequences of&nbsp;similar organism for reference.</p>
<h4h5>Annotation</h4h5><p>&nbsp;Genome annotation is giving some biological&nbsp;information to sequences. Main&nbsp;aspects&nbsp;for genome&nbsp;annotation is&nbsp;finding non-protein coding portion, identifying elements on&nbsp;genome&nbsp;and giving information to elements.</p>
<h1>Transcriptomics&nbsp;</h1>
<h1>Proteomics</h1>
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