<br/> <br/> <span style="font-size:14px;"><span style="line-height:107%">'''<span lang="EN-US">Necessity of RNA Sequencing in BBB Models</span>'''</span><br/> <span style="line-height:107%"><span lang="EN-US">Through fluorescence image analysis, I found out that LNPs migrate to the transcytosis pathway. In addition, I checked the difference in transmittance by performing several transcytosis inhibitor treatments.</span></span><br/> <span style="line-height:107%"><span lang="EN-US">Through RNA sequencing, I would like to check whether the permeability has changed due to changes in the genetic level of the cells that actually make up BBB.</span></span><br/> <br/> <span style="line-height:107%">'''<span lang="EN-US">Future Plan</span>'''</span><br/> <span style="line-height:107%"><span lang="EN-US">Taking to the Genomics course, I thought realized that conducting research at various gene levels was is essential to conduct research at a achieving high -levelresearch outcomes. Later, the My goal is to proceed with the RNA sequencing mentioned above to find out uncover the detailed mechanisms of nanoparticle transport that have not yet been foundidentified. To facilitate this, I am eager to collaborate actively with the bioinformatics labs at UNIST for detailed RNA sequencing analysis. After finding out discovering the mechanismmechanisms, I will plan to treat the Aptamer on surface of the surface nanoparticles with Aptamers to find out the change observe changes in the permeability and put the ultimately encapsulate actual drug inside to deliver itdrugs for targeted delivery.</span><br/span><br/> <br/> <br/span style="font-size:14px;"> <span style="line-height:107%">'''<span lang="EN-US">References</span>'''</span><br/> <span style="line-height:107%"><span lang="EN-US" style="line-height:107%">(1) Cecchelli, Romeo, et al., Modelling of the blood–brain barrier in drug discovery and development., '''2007''', ''Nature Reviews Drug Discovery''. vol. 6, no. 8, pp. 650–661,.</span></span><br/> <br/> <span style="line-height:107%"><span lang="EN-US" style="line-height:107%">(2) Park, Tae-Eun, et al., Hypoxia-enhanced blood-brain barrier chip recapitulates human barrier function and shuttling of drugs and antibodies., '''2019''', ''Nature Communications'', vol. 10, no. 1, 13</span></span><br/> <br/> <span style="line-height:107%"><span lang="EN-US" style="line-height:107%">(3) Abbott, N. Joan, Lars Rönnbäck, and Elisabeth Hansson., Astrocyte–Endothelial Interactions at the Blood–Brain Barrier., '''2006''', ''Nature Reviews Neuroscience 7'', no. 1: 41–53.</span></span><br/> <br/> <span style="line-height:107%"><span lang="EN-US" style="line-height:107%">(4) Mehta, M., Bui, T. A., Yang, X., Aksoy, Y., Goldys, E. M., & Deng, W., Lipid-based nanoparticles for drug/gene delivery: An overview of the production techniques and difficulties encountered in their industrial development., '''2023''', ''ACS Materials Au'', 3(6), 600–619.</span></span><br/> <br/> <span style="line-height:107%"><span lang="EN-US">(5)</span> <span lang="EN-US">Deshpande, Dhrithi, et al., RNA-seq data science: From RAW data to effective interpretation.,'''2023''', ''Frontiers in Genetics'', vol. 14,</span></span></span><br/> &nbsp;