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Created page with " Fluorescent In Situ Hybridization(FISH) DNA sequencing utilizing fluorescent markers is a method to analyze genetic sequences and visualize specific genes or genomic regions..."
Fluorescent In Situ Hybridization(FISH)
DNA sequencing utilizing fluorescent markers is a method to analyze genetic sequences and visualize specific genes or genomic regions by attaching fluorescent dyes to specific bases. One of the most representative methods includes the following procedure:
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'''Use of Fluorescent Markers:''' Each DNA base (A, T, G, C) is tagged with a different fluorescent marker (color). As a result, each base emits light of a specific wavelength, giving it a unique color.
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'''Sequencing Process:''' During DNA sequencing, the sequence of a specific DNA fragment is determined. Each DNA base amplified or replicated in the sequencing reaction is identified by the color of light emitted by the fluorescent marker attached to it.
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'''Image Capture and Analysis:''' The light emitted by the fluorescent markers is captured using a special camera or detector. This data is analyzed by computer software, determining the DNA sequence based on the colors of the fluorescent markers observed at each position.
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'''Sequencing Results:''' Ultimately, the DNA sequence is represented by the order of fluorescent colors. This allows researchers to identify specific areas of the genome, genetic mutations, or genetic changes related to diseases.
<br/> <br/> ref: [https://www.youtube.com/watch?v=MvuYATh7Y74 https://www.youtube.com/watch?v=MvuYATh7Y74]