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<p style="margin-left: 38pt"> Correspondence: <a href="mailto:woobin512@unist.ac.kr">woobin512@unist.ac.kr</a></p>
<p> </p>
<p style="margin-left: 38pt"> </p>
<p><strong>4.1. Result Data </strong></p>
<p> [[File:Self4.png|400px]][[File:Self5.png|400px]]</p>
<p><strong>Figure 2.a. Cloning of pRA-GFP-pRL-mETP-SpyTag-Stop-Xho1 (mammalian expression vector) and its sequencing result. (biolecture_20131571_woobeen_Jo)</strong></p>
<p><strong>Table 3</strong>. Details of cloning pRA_mETP-SpyTag plasmid <strong>(biolecture_20131571_</strong><strong>조우빈)</strong></p>
<p> [[File:Self6.png|400px]][[File:Self7.png|400px]]</p>
<p><strong>Figure 2.b. Cloning of pTrc-Spe1-SpyCatcher-Xho1-APEX2 (E.Coli expression vector) and its sequencing data.</strong></p>
<p>By digesting mETP form Spe1-mETP-Xho1-APEX2 and ligation of SpyCatcher between two restriction enzyme site (Spe1, Xho1), we made pTrc-Spe1-SpyCatcher-Xho1-APEX2 plasmid and confirmed by either double digestion using Spe1, Xho1 restriction enzyme or sequencing data result. <strong>(biolecture_20131571_</strong><strong>조우빈)</strong></p>
<p> [[File:Self8.png|400px]]</p>
<p><strong>Figure 2.c. mETP-SpyTag, SpyCatcher-APEX2 protein binding test</strong></p>