06.03 class

RNA-seq : various techniques for revealing transcriptome - 신재혁
central dogma & transcription
transcription : DNA →  RNA
transcription + -ome = transcriptome
transcription + -omics = transcriptomics
RNA → non-coding RNA (tRNA, rRNA / mRNA, siRNA, snRNA, etc) , coding RNA (mRNA)
sequencing of DNA sequence = information of DNA, Genome analysis
general workflow of RNA-seq
mRNA and Total RNA sequencing follow this workflow
sequencing method is same with DNA-seq → convert RNA to cDNA
appropriate methods to prepare target genes
 - single-cell RNA sequencing
 - RNA exome capture sequencing
 - ribosome profiling
there are multiple types of sequencing method for RNA molecules (because RNA has various type)
transcriptomics is important → most of gene regulation occurs in transcription part
other specific, efficient RNA-seq method is required for further research(currently, RNA → cDNA by reverse transcriptase sequencing. direct RNA sequencing would be better)
 
dogma - something that cennot be wrong / absolute /
central dogma is wrong!!

transcriptomics - 채신혁

trannscriptomics

expression relfects the ups and downs of gene products - correlation between dots (quantity)
Expression 1. RNA expression 2. protein expression
mRNA, tRNA, rRNA, ncRNA, microRNA
" alternative splicing " - specific feature of RNA
horse gens ru
set the groups (1. before/after exercise 2. some horses that do not exercise well)
we sequenced the whole mRNA from the blood and muscels tissues of six throughbred horses before and after exercise
Even though the gene is the same, transcription level can change before and after exercise by alternative splicing