DNA sequence analysis

ORF Finder

Open reading frame is the nucleotide sequence that encodes a protein from start site to finish site

We can find open reading frame

2. Click 'Resource List (A-Z)'

3. Click Open Reading Frame Finder (ORF Finder)

4. Input accession number or nucleotide sequence that you want to analyze (You can get the number or sequence from ncbi FASTA format)

Ex) E.coli GST gene accession number : D38497

5. Click 'OrfFinder'

6. The result is like this

You can see that there are five ORFs. And for the first frame (+1), the length is 198 nutleotide, which encodes 65 amino acids.

You can check each ORF by clicking it

1. Go to the website, molbiol-tools.ca/Promoters.htm

2. Click 'Promoter Prediction by Nueral Network'

3. Choose the type of organism, reverse strand, minimum promoter score(0.8 is default)

4. Input DNA sequence

Ex) Human Helper T Cell Selective Function Controling Method sequence

5. Submit

6. The result is like this