ShinUnBeom

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Genomics

 Genomics is discipline of genetics for sequencing, assembling and analyzing the function and structure of entire genomes, not single genes.

Genome analysis

DNA sequencing

 First of all, select an organism of interest. Then sequence DNA fragments. There are several ways for sequencing DNA fragments; shotgun sequencing[1], high-throughout sequencing, illumina sequencing[2] and ion torrent[3].

Sequence assembly

 After sequencing DNA fragments, next step is assembling DNA fragments to rebuild original chromosome. There are two ways; de-novo assembly[4] which assemble without any reference sequences, and comparative assembly which use sequences of similar organism for reference.

Annotation

 Genome annotation is giving some biological information to sequences. Main aspects for genome annotation is finding non-protein coding portion, identifying elements on genome and giving information to elements.