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What is sequencing?

In genetics and biochemistry, sequencing means to determine the primary structure (sometimes falsely called primary sequence) of an unbranched biopolymer. Sequencing results in a symbolic linear depiction known as a sequence which succinctly summarizes much of the atomic-level structure of the sequenced molecule. DNA sequencing is the process of determining the nucleotide order of a given DNA fragment.

 

Sanger Sequencing

 

 

Shotgun Sequencing

 

Next Generation Sequencing