About sequencing

Sequencing is consist of three steps

 

1. Library preparation

2. Template preparation

3. Sequencing and imaging

 

Library preparation

A genomic library is a collection of the total genomic DNA from a single  organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size.

 

 

As shown as 'Good fragment', fragments are expected to have adaptor(blue colored zone in the above picture) allowing fragments to attached to emPCR bead and solid phase which function for the template preparation .

 

 

Template preparation

 

 

Sequencing and imaging