Difference between revisions of "Dahyun week12"
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DNA sequencing utilizing fluorescent markers is a method to analyze genetic sequences and visualize specific genes or genomic regions by attaching fluorescent dyes to specific bases. One of the most representative methods includes the following procedure: | DNA sequencing utilizing fluorescent markers is a method to analyze genetic sequences and visualize specific genes or genomic regions by attaching fluorescent dyes to specific bases. One of the most representative methods includes the following procedure: | ||
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| − | + | #'''Use of Fluorescent Markers:''' Each DNA base (A, T, G, C) is tagged with a different fluorescent marker (color). As a result, each base emits light of a specific wavelength, giving it a unique color. | |
| − | + | #'''Sequencing Process:''' During DNA sequencing, the sequence of a specific DNA fragment is determined. Each DNA base amplified or replicated in the sequencing reaction is identified by the color of light emitted by the fluorescent marker attached to it. | |
| − | '''Use of Fluorescent Markers:''' Each DNA base (A, T, G, C) is tagged with a different fluorescent marker (color). As a result, each base emits light of a specific wavelength, giving it a unique color. | + | #'''Image Capture and Analysis:''' The light emitted by the fluorescent markers is captured using a special camera or detector. This data is analyzed by computer software, determining the DNA sequence based on the colors of the fluorescent markers observed at each position. |
| − | + | #'''Sequencing Results:''' Ultimately, the DNA sequence is represented by the order of fluorescent colors. This allows researchers to identify specific areas of the genome, genetic mutations, or genetic changes related to diseases. | |
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| − | '''Sequencing Process:''' During DNA sequencing, the sequence of a specific DNA fragment is determined. Each DNA base amplified or replicated in the sequencing reaction is identified by the color of light emitted by the fluorescent marker attached to it. | ||
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| − | # | ||
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| − | '''Image Capture and Analysis:''' The light emitted by the fluorescent markers is captured using a special camera or detector. This data is analyzed by computer software, determining the DNA sequence based on the colors of the fluorescent markers observed at each position. | ||
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| − | # | ||
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| − | '''Sequencing Results:''' Ultimately, the DNA sequence is represented by the order of fluorescent colors. This allows researchers to identify specific areas of the genome, genetic mutations, or genetic changes related to diseases. | ||
<br/> <br/> ref: [https://www.youtube.com/watch?v=MvuYATh7Y74 https://www.youtube.com/watch?v=MvuYATh7Y74] | <br/> <br/> ref: [https://www.youtube.com/watch?v=MvuYATh7Y74 https://www.youtube.com/watch?v=MvuYATh7Y74] | ||
Revision as of 16:32, 25 March 2024
Fluorescent In Situ Hybridization(FISH)
DNA sequencing utilizing fluorescent markers is a method to analyze genetic sequences and visualize specific genes or genomic regions by attaching fluorescent dyes to specific bases. One of the most representative methods includes the following procedure:
- Use of Fluorescent Markers: Each DNA base (A, T, G, C) is tagged with a different fluorescent marker (color). As a result, each base emits light of a specific wavelength, giving it a unique color.
- Sequencing Process: During DNA sequencing, the sequence of a specific DNA fragment is determined. Each DNA base amplified or replicated in the sequencing reaction is identified by the color of light emitted by the fluorescent marker attached to it.
- Image Capture and Analysis: The light emitted by the fluorescent markers is captured using a special camera or detector. This data is analyzed by computer software, determining the DNA sequence based on the colors of the fluorescent markers observed at each position.
- Sequencing Results: Ultimately, the DNA sequence is represented by the order of fluorescent colors. This allows researchers to identify specific areas of the genome, genetic mutations, or genetic changes related to diseases.
