Difference between revisions of "About sequencing"
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<p><span style="color:#000000">A genomic library is a collection of the total genomic DNA</span><span style="color:#000000"> from a single organism</span><span style="color:#000000">. The DNA is stored in a population of identical vectors,</span><span style="color:#000000"> each containing a different insert </span><span style="color:#000000">of DNA. In order to construct a genomic library, the organism's DNA is extracted </span><span style="color:#000000">from cells </span><span style="color:#000000">and then digested with a restriction enzyme </span><span style="color:#000000">to cut the DNA into fragments of a specific size.</span></p> | <p><span style="color:#000000">A genomic library is a collection of the total genomic DNA</span><span style="color:#000000"> from a single organism</span><span style="color:#000000">. The DNA is stored in a population of identical vectors,</span><span style="color:#000000"> each containing a different insert </span><span style="color:#000000">of DNA. In order to construct a genomic library, the organism's DNA is extracted </span><span style="color:#000000">from cells </span><span style="color:#000000">and then digested with a restriction enzyme </span><span style="color:#000000">to cut the DNA into fragments of a specific size.</span></p> | ||
Revision as of 20:02, 4 October 2017
Sequencing is consist of three steps
= Library preparation =
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size.
As shown as 'Good fragment', fragments are expected to have adaptor(blue colored zone in the above picture) allowing fragments to attached to emPCR bead and solid phase which function for the template preparation .
Template preparation
Sequencing and imaging
