Difference between revisions of "Lecture 5 - Proteomics & Epigenomics"
imported>YoungKwang Jung |
imported>YoungKwang Jung |
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Interaction proteomics => protein -protein association<br /> | Interaction proteomics => protein -protein association<br /> | ||
Protein family - protein mother, father</p> | Protein family - protein mother, father</p> | ||
| + | |||
| + | <p> </p> | ||
<p>The first person who identify the sequence of amino acid in protein. - Sanger(insulin)</p> | <p>The first person who identify the sequence of amino acid in protein. - Sanger(insulin)</p> | ||
| + | |||
| + | <p> </p> | ||
<p>Pfam - the data base of protein family<br /> | <p>Pfam - the data base of protein family<br /> | ||
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interproscan<br /> | interproscan<br /> | ||
NCBI NR(non-redundant) db ->largest data base -> check the redundancy</p> | NCBI NR(non-redundant) db ->largest data base -> check the redundancy</p> | ||
| + | |||
| + | <p> </p> | ||
<p>The level of any protein in the cell of <br /> | <p>The level of any protein in the cell of <br /> | ||
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3. the rate of degradation into half life of mRNA</p> | 3. the rate of degradation into half life of mRNA</p> | ||
| − | <p>In my opinion, the number of possilble kinds of protein is 20^300(The number of type of amino acid is 20, and the average length of protein is 300aa ->20*20*20*......*20 ) -> protein space</p> | + | <p> </p> |
| + | |||
| + | <p><strong>In my opinion, the number of possilble kinds of protein is 20^300(The number of type of amino acid is 20, and the average length of protein is 300aa ->20*20*20*......*20 ) -> protein space</strong></p> | ||
| + | |||
| + | <p> </p> | ||
<p>The number of protein types -> about 100,000 <br /> | <p>The number of protein types -> about 100,000 <br /> | ||
The number of type of protein structure -> 4000~1000</p> | The number of type of protein structure -> 4000~1000</p> | ||
| + | |||
| + | <p> </p> | ||
<p>secondary structure - alpha helix/ beta sheet</p> | <p>secondary structure - alpha helix/ beta sheet</p> | ||
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End point of polypeptide - C -terminus</p> | End point of polypeptide - C -terminus</p> | ||
| − | <p>domain - area/region of protein< | + | <p>domain - area/region of protein</p> |
| − | determining protein structure<br /> | + | |
| + | <p>determining protein structure<br /> | ||
1. X-ray crystallography<br /> | 1. X-ray crystallography<br /> | ||
2. NMR -> sensor using magnetic radiation<br /> | 2. NMR -> sensor using magnetic radiation<br /> | ||
Revision as of 00:17, 9 June 2017
<Proteomics & Epigenomics>
Interaction proteomics => protein -protein association
Protein family - protein mother, father
The first person who identify the sequence of amino acid in protein. - Sanger(insulin)
Pfam - the data base of protein family
PDB - protein data bank
scop - classification
swiss porf - protein data base from swiss
UNIPROT - protein data base
interproscan
NCBI NR(non-redundant) db ->largest data base -> check the redundancy
The level of any protein in the cell of
1. rate of transcription of the gene
2. the efficiency of translation of mRNA into protein
3. the rate of degradation into half life of mRNA
In my opinion, the number of possilble kinds of protein is 20^300(The number of type of amino acid is 20, and the average length of protein is 300aa ->20*20*20*......*20 ) -> protein space
The number of protein types -> about 100,000
The number of type of protein structure -> 4000~1000
secondary structure - alpha helix/ beta sheet
Starting point of polypeptide - N - terminus
End point of polypeptide - C -terminus
domain - area/region of protein
determining protein structure
1. X-ray crystallography
2. NMR -> sensor using magnetic radiation
3. protein prediction program - computer
structure & function
Hydrophobicity is determined by the primary and secondary structure
sequence-(not 1:1)- structure -(1:1)- fuction
There is no method to analyze the amino acid sequence
So we use indirect way to the protein such as Mass spectrometry, 2D gel electrophoresis, 2 type of MS, MALDI-TOF
