Difference between revisions of "ShinUnBeom"
imported>ShinUnBeom |
imported>ShinUnBeom |
||
| Line 21: | Line 21: | ||
<h1>Transcriptomics </h1> | <h1>Transcriptomics </h1> | ||
<div> Transcriptomics is a study on RNAs, such as mRNA, rRNA, tRNA and non-coding RNA. Usually transcriptomics examine the expression level of RNA and study the function of RNA.</div> | <div> Transcriptomics is a study on RNAs, such as mRNA, rRNA, tRNA and non-coding RNA. Usually transcriptomics examine the expression level of RNA and study the function of RNA.</div> | ||
| − | < | + | <h3>Analyzing methods</h3> |
| − | < | + | <p> There is two analyzing methods; microarrarys and RNA-seq.</p> |
| − | < | + | <h5>Microarrays</h5> |
| − | <p> </p> | + | <p> Microarrays is a method which collect RNA fragments on solid surface. On solid surface, DNAs are fragmented and attached. When RNAs are introduced in surface, RNA is attached to its anti-sense DNA fragments. So people can see which part of DNA(gene) is highly expressed and figure out the function.[https://en.wikipedia.org/wiki/DNA_microarray]</p> |
| + | <h5>RNA-seq</h5> | ||
| + | <p> RNA-seq, also known as RNA sequencing, is a recently method to analyze the amount of RNA in a specific time by using next-generation sequencing. Fore details, see here;[https://en.wikipedia.org/wiki/RNA-Seq][https://en.wikipedia.org/wiki/DNA_sequencing#Next-generation_methods]</p> | ||
<h1>Proteomics</h1> | <h1>Proteomics</h1> | ||
<h1>Epigenomics and Phenomics</h1> | <h1>Epigenomics and Phenomics</h1> | ||
<h1>Canceromics and Geromics</h1> | <h1>Canceromics and Geromics</h1> | ||
<p> </p> | <p> </p> | ||
Revision as of 01:11, 17 June 2015
Contents
Principles of Bioinformatics
Bioprogramming
Genomics
Genomics is discipline of genetics for sequencing, assembling and analyzing the function and structure of entire genomes, not single genes.
Genome analysis
DNA sequencing
First of all, select an organism of interest. Then sequence DNA fragments. There are several ways for sequencing DNA fragments; shotgun sequencing[1], high-throughout sequencing, illumina sequencing[2] and ion torrent[3].
Sequence assembly
After sequencing DNA fragments, next step is assembling DNA fragments to rebuild original chromosome. There are two ways; de-novo assembly[4] which assemble without any reference sequences, and comparative assembly which use sequences of similar organism for reference.
Annotation
Genome annotation is giving some biological information to sequences. Main aspects for genome annotation is finding non-protein coding portion, identifying elements on genome and giving information to elements.
Fields of genomics
Functional genomics
Functional genomics is a field for studying gene function and interactions. They want to find the relationship between the genome and its phenotype.
Structural genomics
Structural genomics is a field for studying the three dimensional structure of proteins encoded by genome.
Epigenomics
Epigenomics is a study about the complete set of epigenetic modifications, such as DNA methylation and histone modification, in the cell.
Metagenomics
Metagenomics is a study of genetic material of environmental samples.
Transcriptomics
Analyzing methods
There is two analyzing methods; microarrarys and RNA-seq.
Microarrays
Microarrays is a method which collect RNA fragments on solid surface. On solid surface, DNAs are fragmented and attached. When RNAs are introduced in surface, RNA is attached to its anti-sense DNA fragments. So people can see which part of DNA(gene) is highly expressed and figure out the function.[5]
RNA-seq
RNA-seq, also known as RNA sequencing, is a recently method to analyze the amount of RNA in a specific time by using next-generation sequencing. Fore details, see here;[6][7]
Proteomics
Epigenomics and Phenomics
Canceromics and Geromics
