Difference between revisions of "6.10.2016"
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<p> </p> | <p> </p> | ||
| − | <p><strong>Epigenomics | + | <p><strong>Epigenomics</strong></p> |
<p>Epi(outside)</p> | <p>Epi(outside)</p> | ||
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<p>Reversible DNA and Histone modification and RNA interference -> causing chage of gene expression</p> | <p>Reversible DNA and Histone modification and RNA interference -> causing chage of gene expression</p> | ||
| − | <p> | + | <p>histone acetylation/methylation</p> |
| + | |||
| + | <p>Diseases related to the histone modification </p> | ||
| + | |||
| + | <p>How do we study?</p> | ||
| + | |||
| + | <p>1. Bisulfite sequencing : treat cytosine with bisulfite changing it to be uracil ! but leaves methylcytosine unaffected. see with cytosine is changed by sequencing the DNA before and after the bisulfite treatment</p> | ||
| + | |||
| + | <p>2. Nonmethylated specific PCR</p> | ||
| + | |||
| + | <p><strong>Proteomics</strong></p> | ||
| + | |||
| + | <p>Phosphorylation is the switch of proteins. and it requires energy </p> | ||
| + | |||
| + | <p>Proteins - a helix + b sheet for stable structure + coils to bind others</p> | ||
| + | |||
| + | <p>Coils are to bind and to attack.</p> | ||
| + | |||
| + | <p>Glycosylation</p> | ||
| + | |||
| + | <p>Sulfation</p> | ||
| + | |||
| + | <p>How?</p> | ||
| + | |||
| + | <p>1. 2D SDS PAGE</p> | ||
| + | |||
| + | <p>2. Mass spectrometer</p> | ||
| + | |||
| + | <p>Interactome</p> | ||
| − | <p> | + | <p>1. Protein Microarrays</p> |
| − | <p> | + | <p>2. IP</p> |
<p> </p> | <p> </p> | ||
Latest revision as of 15:00, 10 June 2016
presentation by 김건우
Why not in Korea?
He think 'mind' and 'accent' are the casues.
Koreans be afraid to ask question.
He thought that accent affects mind.
'mindset'
Investment on the Basic science
accent can induce racism. asian accent can make the theories not understandable.
Nobel Prize It's all about contribution
Epigenomics
Epi(outside)
Reversible DNA and Histone modification and RNA interference -> causing chage of gene expression
histone acetylation/methylation
Diseases related to the histone modification
How do we study?
1. Bisulfite sequencing : treat cytosine with bisulfite changing it to be uracil ! but leaves methylcytosine unaffected. see with cytosine is changed by sequencing the DNA before and after the bisulfite treatment
2. Nonmethylated specific PCR
Proteomics
Phosphorylation is the switch of proteins. and it requires energy
Proteins - a helix + b sheet for stable structure + coils to bind others
Coils are to bind and to attack.
Glycosylation
Sulfation
How?
1. 2D SDS PAGE
2. Mass spectrometer
Interactome
1. Protein Microarrays
2. IP
