Difference between revisions of "KSH 0608 Bioinformatics with Bioperl"

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imported>Seung-hoon Kim
 
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<p>From today, I will learn and summarize how to use Bioperl&nbsp;by reading a book &quot;Bioinformatics with bioperl (Author: Young-Chang Kim)&quot;</p>
 
<p>From today, I will learn and summarize how to use Bioperl&nbsp;by reading a book &quot;Bioinformatics with bioperl (Author: Young-Chang Kim)&quot;</p>
  
<p>1. Change DNA sequence to RNA sequence</p>
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<p>[[How to install Perl and BioPerl]]</p>
  
<p>$DNASeq = &#39;GATACAA.......&#39;</p>
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<p>[[Basic Perl Grammar]]</p>
  
<p>$RNASeq =~s/T/U/g;</p>
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<p>[[Transcription from DNA sequence to RNA sequence]]</p>
  
<p>=~ : binding operator tells Perl to match the pattern on the right against the string on the left</p>
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<p>[[DATA extraction from FASTA]]</p>
  
<p>(http://docstore.mik.ua/orelly/perl4/lperl/ch09_03.htm)</p>
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<p>[[Motif search]]</p>
  
<p>s/ / / : substitution function, s is substitution operator, / is used to make a distinction</p>
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<p>[[Getting DNA information from NCBI]]</p>
  
<p>g : pattern modifier from start to end</p>
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<p>[[DNA sequence analysis]]</p>
  
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
  
<p>==&gt; We can utilize the =~s/ / /g to the first homework</p>
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<p>[[Seung-hoon Kim]]</p>
 
 
<p>If there is a certain DNA sequence, we can change the sequence into matching amino acid sequence</p>
 
 
 
<p>For example, Methionine has AUG codon sequence and its template DNA sequence is 3&#39;-TAC-5&#39;.</p>
 
 
 
<p>Thus, if we change the CAT sequence into M or Met, we could find the methionine from DNA sequence.</p>
 
 
 
<p>&nbsp;Ex) $Methionine = ~s/CAT/M/g;</p>
 
 
 
<p>Likewise, we could derive whole amino acids from DNA sequence.</p>
 
  
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>
  
 
<p>&nbsp;</p>
 
<p>&nbsp;</p>

Latest revision as of 19:52, 17 June 2016

From today, I will learn and summarize how to use Bioperl by reading a book "Bioinformatics with bioperl (Author: Young-Chang Kim)"

How to install Perl and BioPerl

Basic Perl Grammar

Transcription from DNA sequence to RNA sequence

DATA extraction from FASTA

Motif search

Getting DNA information from NCBI

DNA sequence analysis

 

Seung-hoon Kim