Difference between revisions of "16. 06. 03"
imported>Mi Rae Yeo |
imported>Mi Rae Yeo |
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| − | <p>* | + | <p>* Pre-class<br /> |
1. What is transcriptomics?</p> | 1. What is transcriptomics?</p> | ||
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<p>After this class, I had a question. Is the cDNA is important in transcriptomics? Why cDNA is used?</p> | <p>After this class, I had a question. Is the cDNA is important in transcriptomics? Why cDNA is used?</p> | ||
| − | <p>The answer is yes. For example, to using microarray, complementary sequences for mRNA is needed to attach and analyze the mRNA. cDNA is in complementary relations with mRNA, hence, cDNA is important. However, not using cDNA technique is also exist, such as single molecule Direct RNA Sequencing.</p> | + | <p>The answer is yes. For example, to using microarray, complementary sequences for mRNA is needed to attach and analyze the target mRNA. cDNA is in complementary relations with mRNA, hence, cDNA is important. However, not using cDNA technique is also exist, such as single molecule Direct RNA Sequencing.</p> |
| + | |||
| + | <p>Then, Why don't use just DNA sequence instead of cDNA?</p> | ||
| + | |||
| + | <p>I guess, it's because DNA is consist of both introns and exons, but mRNA is just consist of exons, usually.</p> | ||
<p>In the Jong’s PPT, there was slide about life. I was very impressed about that slide when I saw it first, so I thought about life. In my definition, life is linkage. People are linked with other person or not, like enzymes binds with substrates or not.</p> | <p>In the Jong’s PPT, there was slide about life. I was very impressed about that slide when I saw it first, so I thought about life. In my definition, life is linkage. People are linked with other person or not, like enzymes binds with substrates or not.</p> | ||
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<p>*Homework</p> | <p>*Homework</p> | ||
| − | <p> - Essay (Genome | + | <p> - <a href="http://biolecture.org/index.php/Essay_(What_is_the_benefits_and_costs_of_the_Genome_Analysis_Service%3F)">Essay (What is the benefits and costs of the Genome Analysis Service?)</a></p> |
| − | <p> - Essay (Why | + | <p> - <a href="http://biolecture.org/index.php/Essay_-_Why_Korean_students_are_so_quiet_in_class%3F" title="Essay - Why Korean students are so quiet in class?"><span style="color:#0000FF">Essay (Why Korean students are so quiet in class?</span></a>)</p> |
<p> </p> | <p> </p> | ||
Latest revision as of 19:17, 17 June 2016
* Pre-class
1. What is transcriptomics?
: study about expression level of transcriptome, which is a collection of all the messenger RNA in a particular cell.
(Source: http://www.collinsdictionary.com/dictionary/english/transcriptome, http://terms.naver.com/entry.nhn?docId=2836479&cid=42411&categoryId=42411)
2. Relationship between genomics and transcriptomics
: genomics are focusing on the DNA, on the other hands, transcriptomics are focusing on the RNA.
(# genomics: the branch of molecular genetics concerned with the study of genomes, specifically the identification and sequencing of their constituent genes and the application of this knowledge in medicine, pharmacy, agriculture, etc.)
(Source: http://www.collinsdictionary.com/dictionary/english/genomics)
3. What are mRNAs?
: RNA, synthesized from a DNA template during transcription, that mediates the transfer of genetic information from the cell nucleus to ribosomes in the cytoplasm, where it serves as a template for protein synthesis.
(Source: http://www.dictionary.com/browse/mrna)
4. How to measure RNA expression?
: By using techniques which are Microarray and RNA-seq.
5. Relationship between Transcriptome and Proteome.
: Transcriptome is the initial product of genome expression, whereas Proteome is the final product of genome expression.
(Source: http://www.ncbi.nlm.nih.gov/books/NBK21121)
6. What is a UTR?
: UTR is untranslated region, which is located at the both ends of mRNA.
(Source: https://en.wikipedia.org/wiki/Untranslated_region)
7. What is ncRNA ?
: ncRNA is a non-coding RNA, which is functional RNA molecule that is transcribed but not translated.
(Source: http://www.whatisepigenetics.com/non-coding-rna/#prettyPhoto)
After this class, I had a question. Is the cDNA is important in transcriptomics? Why cDNA is used?
The answer is yes. For example, to using microarray, complementary sequences for mRNA is needed to attach and analyze the target mRNA. cDNA is in complementary relations with mRNA, hence, cDNA is important. However, not using cDNA technique is also exist, such as single molecule Direct RNA Sequencing.
Then, Why don't use just DNA sequence instead of cDNA?
I guess, it's because DNA is consist of both introns and exons, but mRNA is just consist of exons, usually.
In the Jong’s PPT, there was slide about life. I was very impressed about that slide when I saw it first, so I thought about life. In my definition, life is linkage. People are linked with other person or not, like enzymes binds with substrates or not.
*Homework
- Essay (What is the benefits and costs of the Genome Analysis Service?)
- Essay (Why Korean students are so quiet in class?)
