Difference between revisions of "Trancriptomics Assay"
From Biolecture.org
imported>S |
imported>S |
||
| (4 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
<p> </p> | <p> </p> | ||
| − | < | + | <p>[[Youngjun Bhak]]</p> |
| − | + | <h2><span style="font-size: 13.5pt; color: black; sans-serif: "> What is transcriptomics?</span></h2> | |
| − | |||
| − | |||
| − | |||
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Thranscriptomics is discipline of biology that study about the set of all RNA moleculs, including mRNA, rRNA, tRNA and non-coding RNA transcribed in one cell or a population of cells</span></p> | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Thranscriptomics is discipline of biology that study about the set of all RNA moleculs, including mRNA, rRNA, tRNA and non-coding RNA transcribed in one cell or a population of cells</span></p> | ||
| − | < | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> |
| − | < | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> |
| − | + | <h2><span style="font-size: 13.5pt; color: black; sans-serif: ">Relationship between genomics and transcriptomics</span></h2> | |
| − | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> | |
| − | |||
| − | </ | ||
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Transcriptomics is expressional genomics. </span></p> | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Transcriptomics is expressional genomics. </span></p> | ||
| − | < | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> |
| − | < | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> |
| − | + | <h2><span style="font-size: 13.5pt; color: black; sans-serif: ">What are mRNAs?</span></h2> | |
| − | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> | |
| − | |||
| − | </ | ||
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">mRNA is large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence if the protein products of gene expressing</span></p> | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">mRNA is large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence if the protein products of gene expressing</span></p> | ||
| − | < | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> |
| − | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> | |
| − | < | + | <h2><span style="font-size: 13.5pt; color: black; sans-serif: ">How to measure RNA expression?</span></h2> |
| − | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; sans-serif: ">RNA-seq method is frequently used for measuring RNA expression.</span></p> | |
| − | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> | |
| − | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> | |
| − | + | <h2><span style="font-size: 13.5pt; color: black; sans-serif: ">Relationship between Transcriptome and Proteome.</span></h2> | |
| − | <p style="background: white; margin: 4.8pt 0cm 6pt | ||
| − | < | ||
| − | < | ||
| − | < | ||
| − | |||
| − | |||
| − | |||
| − | |||
<div style="background: white; margin: 4.8pt 0cm 6pt; line-height: 14.3pt; text-indent: 20.25pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Proteome is expressional Transcriptome. </span></div> | <div style="background: white; margin: 4.8pt 0cm 6pt; line-height: 14.3pt; text-indent: 20.25pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Proteome is expressional Transcriptome. </span></div> | ||
| − | <div style="margin: 0cm | + | <div style="background: white; margin: 4.8pt 0cm 6pt; line-height: 14.3pt; text-indent: 20.25pt"> </div> |
| − | <div style="background: white; margin: 4.8pt 0cm 6pt | + | <div style="background: white; margin: 4.8pt 0cm 6pt; line-height: 14.3pt; text-indent: 20.25pt"> </div> |
| − | < | + | <h2><span style="font-size: 13.5pt; color: black; sans-serif: ">What is a UTR?</span></h2> |
| − | + | <div style="background: white; margin: 4.8pt 0cm 6pt; line-height: 14.3pt; text-indent: 20.25pt"> </div> | |
| − | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; background: white; sans-serif: ">Untranslated region </span><span style="font-size: 13.5pt; background: white; sans-serif: ">(or UTR) refers to either of two sections, one on each side of a </span><a title="Coding sequence" href="http://en.wikipedia.org/wiki/Coding_sequence"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">coding sequence</span> <span style="font-size: 13.5pt; background: white; sans-serif: ">on a strand of </span></a><a title="MRNA" href="http://en.wikipedia.org/wiki/MRNA"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">mRNA</span><span style="font-size: 13.5pt; background: white; sans-serif: ">. If it is found on the 5' side, it is called the </span></a><a title="Five prime untranslated region" href="http://en.wikipedia.org/wiki/Five_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">5' UTR</span> <span style="font-size: 13.5pt; background: white; sans-serif: ">(or leader sequence), or if it is found on the 3' side, it is called the </span></a><a title="Three prime untranslated region" href="http://en.wikipedia.org/wiki/Three_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">3' UTR</span> <span style="font-size: 13.5pt; background: white; sans-serif: ">(or trailer sequence).</span></a></p> | |
| − | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> | |
| − | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> | |
| − | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; background: white; sans-serif: ">Untranslated region </span><span style="font-size: 13.5pt; background: white; sans-serif: ">(or UTR) refers to either of two sections, one on each side of a </span><a title="Coding sequence" href="http://en.wikipedia.org/wiki/Coding_sequence"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">coding sequence</span> <span style="font-size: 13.5pt; background: white; sans-serif: ">on a strand of </span><a title="MRNA" href="http://en.wikipedia.org/wiki/MRNA"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">mRNA</span><span style="font-size: 13.5pt; background: white; sans-serif: ">. If it is found on the 5' side, it is called the </span><a title="Five prime untranslated region" href="http://en.wikipedia.org/wiki/Five_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">5' UTR</span> <span style="font-size: 13.5pt; background: white; sans-serif: ">(or leader sequence), or if it is found on the 3' side, it is called the </span><a title="Three prime untranslated region" href="http://en.wikipedia.org/wiki/Three_prime_untranslated_region"><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; text-underline: none; sans-serif: ">3' UTR</span> <span style="font-size: 13.5pt; background: white; sans-serif: ">(or trailer sequence).</span | + | <h2><span style="font-size: 13.5pt; color: black; sans-serif: ">What is ncRNA ?</span></h2> |
| − | < | + | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"> </p> |
| − | < | + | <div style="margin: 0cm 0cm 8pt 15pt"><span style="font-size: 13.5pt; background: white; line-height: 107%; sans-serif: ">A non-coding RNA (ncRNA) is a functional </span><u><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; line-height: 107%; text-underline: none; sans-serif: ">RNA Molecule that is not<font color="#0066cc"> </font></span></u><a title="Translation (genetics)" href="http://en.wikipedia.org/wiki/Translation_(genetics)"><u><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; line-height: 107%; text-underline: none; sans-serif: ">translated</span><span style="font-size: 13.5pt; background: white; line-height: 107%; sans-serif: "> into a </span></u></a><a title="Protein" href="http://en.wikipedia.org/wiki/Protein"><u><span style="font-size: 13.5pt; text-decoration: none; background: white; color: windowtext; line-height: 107%; text-underline: none; sans-serif: ">protein</span></u></a></div> |
| − | |||
| − | |||
| − | |||
| − | </ | ||
| − | <div style="margin: 0cm 0cm 8pt 15pt"><span style="font-size: 13.5pt; background: white; line-height: 107%; sans-serif: ">A non-coding RNA (ncRNA) is a functional </span>< | ||
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div> | <div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div> | ||
<div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div> | <div style="background: white; margin: 4.8pt 0cm 6pt 38pt; line-height: 14.3pt"> </div> | ||
| Line 62: | Line 42: | ||
<p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Poly A is long tail sequence of adenine nucleotides add it the 3’end of the pre-mRNA. This promotes export from the nucleus and translation, and protects the mRNA from degradation</span></p> | <p style="background: white; margin: 4.8pt 0cm 6pt 20pt; line-height: 14.3pt"><span style="font-size: 13.5pt; color: black; sans-serif: ">Poly A is long tail sequence of adenine nucleotides add it the 3’end of the pre-mRNA. This promotes export from the nucleus and translation, and protects the mRNA from degradation</span></p> | ||
<div style="margin: 0cm 0cm 8pt"><font size="2"> </font></div> | <div style="margin: 0cm 0cm 8pt"><font size="2"> </font></div> | ||
| + | <p> </p> | ||
Latest revision as of 00:41, 21 May 2015
Contents
What is transcriptomics?
Thranscriptomics is discipline of biology that study about the set of all RNA moleculs, including mRNA, rRNA, tRNA and non-coding RNA transcribed in one cell or a population of cells
Relationship between genomics and transcriptomics
Transcriptomics is expressional genomics.
What are mRNAs?
mRNA is large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence if the protein products of gene expressing
How to measure RNA expression?
RNA-seq method is frequently used for measuring RNA expression.
Relationship between Transcriptome and Proteome.
Proteome is expressional Transcriptome.
What is a UTR?
Untranslated region (or UTR) refers to either of two sections, one on each side of a coding sequence on a strand of mRNA. If it is found on the 5' side, it is called the 5' UTR (or leader sequence), or if it is found on the 3' side, it is called the 3' UTR (or trailer sequence).
What is ncRNA ?
-
What is poly A?
Poly A is long tail sequence of adenine nucleotides add it the 3’end of the pre-mRNA. This promotes export from the nucleus and translation, and protects the mRNA from degradation
