Difference between revisions of "Epigenomics-OG"
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imported>Gun Oh (Created page with "<p>Epigenomics</p> <p> </p> <p>"Epi">>reversible</p> <p> </p> <p>definition : modifications on DNA /histones</p> <ul> <li>alter the DNA ...") |
imported>Gun Oh |
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<p> </p> | <p> </p> | ||
| + | |||
| + | <p>feature</p> | ||
| + | |||
| + | <ul> | ||
| + | <li>regulate gene expression : turn on / off </li> | ||
| + | <li>not part of a DNA sequence , but in some cases , they can be inherited to offspring</li> | ||
| + | <li>In general, methylation turn off the gene expression while acetylation turn on the gene expression</li> | ||
| + | </ul> | ||
<p> </p> | <p> </p> | ||
| − | <p> | + | <p>histone modification</p> |
<ul> | <ul> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li>DNA acetylation</li> | ||
| + | <li>DNA phosphorylation</li> | ||
| + | <li>DNA ubiquitination</li> | ||
| + | <li>SUMOylation</li> | ||
| + | <li>ADP-ribosylation</li> | ||
| + | <li>deamination</li> | ||
| + | </ul> | ||
| + | |||
| + | <p> </p> | ||
| + | |||
| + | <p>How to detect histone modification?</p> | ||
| + | |||
| + | <ul> | ||
| + | <li>Chip-Chip : chromatin immunoprecipitation with DNA microarrays | ||
| + | <ul> | ||
| + | <li>immunoprecipitation : specific antibodies are used to immunoprecipitate the DNA-histone complexes</li> | ||
| + | <li>visualization : purify immunoprecipitation --> amplification --> labeled with fluorescent tag</li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
</ul> | </ul> | ||
| + | |||
| + | <p> </p> | ||
| + | |||
| + | <hr /> | ||
| + | <p>reference</p> | ||
| + | |||
| + | <p>1) https://en.wikipedia.org/wiki/Epigenomics</p> | ||
| + | |||
| + | <p>2) https://ghr.nlm.nih.gov/primer/howgeneswork/epigenome</p> | ||
Latest revision as of 15:56, 19 June 2016
"Epi">>reversible
definition : modifications on DNA /histones
- alter the DNA sequence
- Tumorigenesis : cancer --> expression / regulation change
feature
- regulate gene expression : turn on / off
- not part of a DNA sequence , but in some cases , they can be inherited to offspring
- In general, methylation turn off the gene expression while acetylation turn on the gene expression
histone modification
- DNA methylation
-
- DNA methylase (DMNT)
- effect
-
- imprinting : transfer certain environmental factor
- X-chromosome inactivation
- Heterochromatin maintenance
- Developmental controls
- Tissue specific expression controls
- How to sequence methylation?
-
- Biosulfite treatment of cytosine : cytosine --> uracil ( 5-methylcytosine residues is unaffected)
- DNA acetylation
- DNA phosphorylation
- DNA ubiquitination
- SUMOylation
- ADP-ribosylation
- deamination
How to detect histone modification?
- Chip-Chip : chromatin immunoprecipitation with DNA microarrays
- immunoprecipitation : specific antibodies are used to immunoprecipitate the DNA-histone complexes
- visualization : purify immunoprecipitation --> amplification --> labeled with fluorescent tag
reference
