Difference between revisions of "DNA sequence analysis"
imported>Seung-hoon Kim (Created page with "<h2> ORF Finder</h2> <p>Open reading frame is the nucleotide sequence that encodes a protein from start site to finish site</p> <p>We can find open reading frame</p> ...") |
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Revision as of 21:04, 17 June 2016
ORF Finder
Open reading frame is the nucleotide sequence that encodes a protein from start site to finish site
We can find open reading frame
1. Go to ncbi (www.ncbi.nlm.nih.gov)
2. Click 'Resource List (A-Z)'
3. Click Open Reading Frame Finder (ORF Finder)
4. Input accession number or nucleotide sequence that you want to analyze (You can get the number or sequence from ncbi FASTA format)
Ex) E.coli GST gene accession number : D38497
5. Click 'OrfFinder'
6. The result is like this
You can see that there are five ORFs. And for the first frame (+1), the length is 198 nutleotide, which encodes 65 amino acids.
You can check each ORF by clicking it
Promoter prediction site
1. Go to the website, molbiol-tools.ca/Promoters.htm
2. Click 'Promoter Prediction by Nueral Network'
3. Choose the type of organism, reverse strand, minimum promoter score(0.8 is default)
4. Input DNA sequence
Ex) Human Helper T Cell Selective Function Controling Method sequence
5. Submit
6. The result is like this
