Difference between revisions of "6/3 ask"
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| − | <p>Friedrich Miescher : identify the DNA and then RNA</p> | + | <p>4.Chip seq</p> |
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| + | <p>object : sequencing the DNA which interact with certain protein</p> | ||
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| + | <p> </p> | ||
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| + | <p>*Friedrich Miescher : identify the DNA and then RNA</p> | ||
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| + | <p>Q. I think DNA chip and RNA seq are the methods that measure the quantity of the RNA<br /> | ||
| + | is there any method which measures the quality of the RNA<br /> | ||
| + | I mean for example the half-life of RNA. (big database?)</p> | ||
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| + | <p>=> the correlation btw protein level and RNA level in human is lower that in prokaryote. it is more complicate.</p> | ||
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| + | <p>*exercise : change in 30min (RNA which is related with signaling or TF will be a key, not genome), functional protein will be also a key.</p> | ||
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| + | <p>Conclusion : sudden environmental change => check RNA!</p> | ||
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| + | <p>How fast transcription</p> | ||
Latest revision as of 15:16, 3 June 2016
1.DNA microarray
object : hybridize the DNA or cDNA and compare the quantity of subject.
2.RNA seq
object : cDNA->next generation sequencing
3. NGS
object : fast sequencing
- Illumina (Solexa) sequencing
- Roche 454 sequencing
- Ion torrent: Proton / PGM sequencing
- SOLiD sequencing
4.Chip seq
object : sequencing the DNA which interact with certain protein
*Friedrich Miescher : identify the DNA and then RNA
Q. I think DNA chip and RNA seq are the methods that measure the quantity of the RNA
is there any method which measures the quality of the RNA
I mean for example the half-life of RNA. (big database?)
=> the correlation btw protein level and RNA level in human is lower that in prokaryote. it is more complicate.
*exercise : change in 30min (RNA which is related with signaling or TF will be a key, not genome), functional protein will be also a key.
Conclusion : sudden environmental change => check RNA!
How fast transcription
