Difference between revisions of "Trancriptomics Assay"
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Revision as of 00:34, 21 May 2015
1. What is transcriptomics?<o:p></o:p>
Thranscriptomics is discipline of biology that study about the set of all RNA moleculs, including mRNA, rRNA, tRNA and non-coding RNA transcribed in one cell or a population of cells<o:p></o:p>
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2. Relationship between genomics and transcriptomics<o:p></o:p>
Transcriptomics is expressional genomics. <o:p></o:p>
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3. What are mRNAs?<o:p></o:p>
mRNA is large family of RNA molecules that convey genetic information from DNA to the ribosome, where they specify the amino acid sequence if the protein products of gene expressing<o:p></o:p>
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4. How to measure RNA expression?<o:p></o:p>
RNA-seq method is frequently used for measuring RNA expression.<o:p></o:p>
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5. Relationship between Transcriptome and Proteome.<o:p></o:p>
Proteome is expressional Transcriptome. <o:p></o:p>
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6. What is a UTR?<o:p></o:p>
Untranslated region (or UTR) refers to either of two sections, one on each side of a coding sequence on a strand of mRNA. If it is found on the 5' side, it is called the 5' UTR (or leader sequence), or if it is found on the 3' side, it is called the 3' UTR (or trailer sequence).<o:p></o:p>
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7. What is ncRNA ?<o:p></o:p>
A non-coding RNA (ncRNA) is a functional RNA molecule that is not translated into a protein<o:p></o:p>
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8. What is poly A?<o:p></o:p>
Poly A is long tail sequence of adenine nucleotides add it the 3’end of the pre-mRNA. This promotes export from the nucleus and translation, and protects the mRNA from degradation<o:p></o:p>
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