Difference between revisions of "Transcriptomics Class"
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| − | <p><span style="font-size: | + | <p><span style="font-size:22px">[ Transcriptomics class ] </span></p> |
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<p> </p> | <p> </p> | ||
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| − | <p><span style="font-size: medium">What to | + | <p><span style="font-size:16px"># Transcriptome? </span></p> |
| − | <p><span style="font-size: medium"> | + | |
| − | <p><span style="font-size: medium"> | + | <p> = set of all mRNA molecules that are produced by the gemone, under specific circumstances in one cell or a population of cells </p> |
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| + | <p> = can vary depending on environmental conditions</p> | ||
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| + | <p> ⇒ reflects genes which are being actively expressed with the exception of mRNA degradation </p> | ||
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| + | <p><span style="font-size:16px"># <span style="line-height:25.6px">Transcriptomics?</span></span></p> | ||
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| + | <p> = the study of transcriptome </p> | ||
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| + | <p> = referred to as expression profiling</p> | ||
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| + | <p> = examines the expression level of mRNAs in a given cell population, often using high-throughput techniques based on DNA microarray technology. </p> | ||
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| + | <p><span style="font-size:medium">2. Relationship between genomics and transcriptomics</span></p> | ||
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| + | <p><span style="font-size:medium">3. What are mRNAs?</span></p> | ||
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| + | <p><span style="font-size:medium">4. How to measure RNA expression?</span></p> | ||
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| + | <p><span style="font-size:medium">5. Relationship between Transcriptome and Proteome.</span></p> | ||
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| + | <p><span style="font-size:medium">6. What is a UTR?</span></p> | ||
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| + | <p><span style="font-size:medium">7. What is ncRNA ?</span></p> | ||
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| + | <p><span style="font-size:medium">8. What is poly A ?</span></p> | ||
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<p> </p> | <p> </p> | ||
| − | <p><span style="font-size: large">Reference</span></p> | + | |
| + | <p><span style="font-size:medium"> </span></p> | ||
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| + | <p><span style="font-size:medium">What to do:</span></p> | ||
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| + | <p><span style="font-size:medium">1. Apply [[Biology learning procedure]]</span></p> | ||
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| + | <p><span style="font-size:medium"> </span></p> | ||
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| + | <p> </p> | ||
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| + | <p><span style="font-size:large">Reference</span></p> | ||
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<p><a href="http://www.biomedcentral.com/1471-2164/13/473">http://www.biomedcentral.com/1471-2164/13/473</a></p> | <p><a href="http://www.biomedcentral.com/1471-2164/13/473">http://www.biomedcentral.com/1471-2164/13/473</a></p> | ||
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<p>https://www.youtube.com/watch?v=UYJfM2-Y7y8 (Youtube by Illumina Gary Schroth)</p> | <p>https://www.youtube.com/watch?v=UYJfM2-Y7y8 (Youtube by Illumina Gary Schroth)</p> | ||
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<p> </p> | <p> </p> | ||
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<p> </p> | <p> </p> | ||
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<p> </p> | <p> </p> | ||
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<h1>Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNA-Seq</h1> | <h1>Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNA-Seq</h1> | ||
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| − | <p | + | <div class="multipleins"> |
| + | <p><strong>Kyung-Do Park</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins1">1</a></sup><sup>†</sup>, <strong>Jongsun Park</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup><sup>†</sup>, <strong>Junsu Ko</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup><sup>†</sup>, <strong>Byung Chul Kim</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Heui-Soo Kim</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins4">4</a></sup>, <strong>Kung Ahn</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins4">4</a></sup>, <strong>Kyoung-Tag Do</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins5">5</a></sup>, <strong>Hansol Choi</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Hak-Min Kim</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Sanghoon Song</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Sunghoon Lee</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Sungwoong Jho</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup>, <strong>Hong-Sik Kong</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins1">1</a></sup>, <strong>Young Mok Yang</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins6">6</a></sup>, <strong>Byung-Hak Jhun</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins7">7</a></sup>, <strong>Chulhong Kim</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Tae-Hyung Kim</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup>, <strong>Seungwoo Hwang</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins8">8</a></sup>, <strong>Jong Bhak</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins2">2</a></sup><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins3">3</a></sup><sup>*</sup>, <strong>Hak-Kyo Lee</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins1">1</a></sup><sup>*</sup> and <strong>Byung-Wook Cho</strong><sup><a href="http://www.biomedcentral.com/1471-2164/13/473/#ins5">5</a></sup><sup>*</sup></p> | ||
</div> | </div> | ||
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<p> </p> | <p> </p> | ||
Revision as of 18:15, 3 June 2016
[ Transcriptomics class ]
# Transcriptome?
= set of all mRNA molecules that are produced by the gemone, under specific circumstances in one cell or a population of cells
= can vary depending on environmental conditions
⇒ reflects genes which are being actively expressed with the exception of mRNA degradation
# Transcriptomics?
= the study of transcriptome
= referred to as expression profiling
= examines the expression level of mRNAs in a given cell population, often using high-throughput techniques based on DNA microarray technology.
2. Relationship between genomics and transcriptomics
3. What are mRNAs?
4. How to measure RNA expression?
5. Relationship between Transcriptome and Proteome.
6. What is a UTR?
7. What is ncRNA ?
8. What is poly A ?
What to do:
1. Apply Biology learning procedure
Reference
http://www.biomedcentral.com/1471-2164/13/473
https://www.youtube.com/watch?v=UYJfM2-Y7y8 (Youtube by Illumina Gary Schroth)
Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNA-Seq
Kyung-Do Park1†, Jongsun Park2†, Junsu Ko3†, Byung Chul Kim23, Heui-Soo Kim4, Kung Ahn4, Kyoung-Tag Do5, Hansol Choi2, Hak-Min Kim2, Sanghoon Song3, Sunghoon Lee2, Sungwoong Jho2, Hong-Sik Kong1, Young Mok Yang6, Byung-Hak Jhun7, Chulhong Kim3, Tae-Hyung Kim3, Seungwoo Hwang8, Jong Bhak23*, Hak-Kyo Lee1* and Byung-Wook Cho5*
