Difference between revisions of "Method of Sample (gDNA) Preparation for Sending out"
imported>ZHOU YANG |
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| − | <h1><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline"> | + | <h1><span id="1..C2.A0Sample.28gDNA.29_Preparation" class="mw-headline">Sample(gDNA) Preparation</span></h1> |
<p>1) DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.</p> | <p>1) DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.</p> | ||
<p>2) Do Not Dissolve in Water</p> | <p>2) Do Not Dissolve in Water</p> | ||
<p> </p> | <p> </p> | ||
| − | <h1><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline"> | + | <h1><span id="2..C2.A0Sample.28gDNA.29_Requirement" class="mw-headline">Sample(gDNA) Requirement</span></h1> |
<p>1) Amount and concentration spec. : over 20 ug of gDNA with >50 ng/ul</p> | <p>1) Amount and concentration spec. : over 20 ug of gDNA with >50 ng/ul</p> | ||
<p> This amount is required for</p> | <p> This amount is required for</p> | ||
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<p>2) Sample(gDNA) should be : OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</p> | <p>2) Sample(gDNA) should be : OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination</p> | ||
<p> </p> | <p> </p> | ||
| − | <h1><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline"> | + | <h1><span id="3..C2.A0Sample.28gDNA.29_Packing_Information.C2.A0" class="mw-headline">Sample(gDNA) Packing Information </span></h1> |
<p>This way is only available when DNA is dissolved in TE or Tris buffer.</p> | <p>This way is only available when DNA is dissolved in TE or Tris buffer.</p> | ||
<p>1) If not, please pack DNA with icepack in icebox and use Fedex or DHL service</p> | <p>1) If not, please pack DNA with icepack in icebox and use Fedex or DHL service</p> | ||
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<p>6) Send the sample with air cushion envelop by FEDEX or DHL or equivalent</p> | <p>6) Send the sample with air cushion envelop by FEDEX or DHL or equivalent</p> | ||
<p> </p> | <p> </p> | ||
| − | <h1><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline"> | + | <h1><span id="4..C2.A0Requirement_of_Sample.28gDNA.29_Information" class="mw-headline">Requirement of Sample(gDNA) Information</span></h1> |
<p>1) Sample type: DNA</p> | <p>1) Sample type: DNA</p> | ||
<p>2) Condition: DNA in TE buffer or EB buffer or equivalent. Not in water</p> | <p>2) Condition: DNA in TE buffer or EB buffer or equivalent. Not in water</p> | ||
Latest revision as of 21:51, 25 November 2011
Contents
Sample(gDNA) Preparation
1) DNA should be dissolved in TE buffer (Tris 10mM pH 8,0, 0.1 mM EDTA) or 10 mM Tris pH 8.0.
2) Do Not Dissolve in Water
Sample(gDNA) Requirement
1) Amount and concentration spec. : over 20 ug of gDNA with >50 ng/ul
This amount is required for
a. Whole genome sequencing (30X)
b. Whole exome sequencing (50X)
c. Genotyping
2) Sample(gDNA) should be : OD260/280=1.8~2.0; free of protein, RNA, or other visible contamination
Sample(gDNA) Packing Information
This way is only available when DNA is dissolved in TE or Tris buffer.
1) If not, please pack DNA with icepack in icebox and use Fedex or DHL service
2) Mark sample information on tube (concentration, sample name…what you want)
3) Seal the cap of tube with parafilm well
4) Wrap the tube with paper towel or equivalent
5) Put into the 50 ml Falcon tube and close the cap
6) Send the sample with air cushion envelop by FEDEX or DHL or equivalent
Requirement of Sample(gDNA) Information
1) Sample type: DNA
2) Condition: DNA in TE buffer or EB buffer or equivalent. Not in water
3) Gel image of DNA electrophoresis
4) Species
5) No. of Tubes
6) Concentration (ng/μl)
7) Volume (μl)
8) Total Quantity (μg)
9) OD260/280
10) OD260/230
Please download and refer to the following documents for information on gDNA methods of preparation and delivery.
